Abstract

IN ADDITION TO THE USE OF POLAROGRAPHIC/VOLTAMMETRIC SYSTEMS FOR THE DETECTION OF OXYGEN AND/OR NITRIC OXIDE FROM SINGLE CELLS WE ARE EXPLORING THE POSSIBILITY OF USING THIS APPROACH FOR THE MEASUREMENT OF PHYSIOLOGICALLY RELEVANT METALS AND TOXIC HEAVY METALS. THE TECHNIQUE IS BASED ON DISCRIMINANTING ION FLUXES BY VIRTUE OF CLOSE REDOX POTENTIALS. HEAVY METALS ARE WIDELY SEPARATED WHEREAS PHYSIOLOGICAL METAL IONS ARE TIGHTLY GROUPED. WE ARE CONFIDENT THAT THE FORMER CAN BE MEASURED AT THE SINGLE CELL LEVEL. DETECTING AND DISCRIMINATING THE LATTER WILL UNDOUBTEDLY BE A GREATER CHALLENGE.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
3P41RR001395-14S1
Application #
5223334
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
14
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Demidenko, Eugene; Glaholt, S P; Kyker-Snowman, E et al. (2017) Single toxin dose-response models revisited. Toxicol Appl Pharmacol 314:12-23
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Van Mooy, Benjamin A S; Hmelo, Laura R; Fredricks, Helen F et al. (2014) Quantitative exploration of the contribution of settlement, growth, dispersal and grazing to the accumulation of natural marine biofilms on antifouling and fouling-release coatings. Biofouling 30:223-36
Brodsky, Alexander S; Fischer, Andrew; Miller, Daniel H et al. (2014) Expression profiling of primary and metastatic ovarian tumors reveals differences indicative of aggressive disease. PLoS One 9:e94476
De Martino, Federico; Zimmermann, Jan; Muckli, Lars et al. (2013) Cortical depth dependent functional responses in humans at 7T: improved specificity with 3D GRASE. PLoS One 8:e60514
De Martino, Federico; Moerel, Michelle; van de Moortele, Pierre-Francois et al. (2013) Spatial organization of frequency preference and selectivity in the human inferior colliculus. Nat Commun 4:1386
Vang, Souriya; Wu, Hsin-Ta; Fischer, Andrew et al. (2013) Identification of ovarian cancer metastatic miRNAs. PLoS One 8:e58226
Chowanadisai, Winyoo; Graham, David M; Keen, Carl L et al. (2013) Neurulation and neurite extension require the zinc transporter ZIP12 (slc39a12). Proc Natl Acad Sci U S A 110:9903-8
Graham, David M; Messerli, Mark A; Pethig, Ronald (2012) Spatial manipulation of cells and organelles using single electrode dielectrophoresis. Biotechniques 52:39-43

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