Abstract

One of the experimental platforms at the Center incorporates a Zeiss Attofluor Ratio Imaging System capable of monitoring intracellular concentrations of ionic calcium and hydrogen. In order, however, to use this system along with the probes, we also need to obtain bright-field images. This allows the investigator to place the electrodes in the cellular near-filed and to ensure that the position remains relatively constant. To achieve this without constantly tripping the high intensity imaging cameras we have designed and installed an LED (light emitting diode) system which mounts directly on the normal lamp housing. A dichroic mirror prevents interference with the normal light path when this is required. The dozen LEDs used generate very little heat and provide ample light when viewed by an infrared sensitive camera, producing an image similar to bright field. IR filters are installed on the two CCD imaging cameras thus allowing simultaneous imaging.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001395-17
Application #
6120175
Study Section
Project Start
1998-12-01
Project End
2000-02-29
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
17
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
Marine Biological Laboratory
Department
Type
DUNS #
001933779
City
Woods Hole
State
MA
Country
United States
Zip Code
02543

  Publications

Demidenko, Eugene; Glaholt, S P; Kyker-Snowman, E et al. (2017) Single toxin dose-response models revisited. Toxicol Appl Pharmacol 314:12-23
Chowanadisai, Winyoo; Messerli, Shanta M; Miller, Daniel H et al. (2016) Cisplatin Resistant Spheroids Model Clinically Relevant Survival Mechanisms in Ovarian Tumors. PLoS One 11:e0151089
De Martino, Federico; Moerel, Michelle; Ugurbil, Kamil et al. (2015) Less noise, more activation: Multiband acquisition schemes for auditory functional MRI. Magn Reson Med 74:462-7
Van Mooy, Benjamin A S; Hmelo, Laura R; Fredricks, Helen F et al. (2014) Quantitative exploration of the contribution of settlement, growth, dispersal and grazing to the accumulation of natural marine biofilms on antifouling and fouling-release coatings. Biofouling 30:223-36
Brodsky, Alexander S; Fischer, Andrew; Miller, Daniel H et al. (2014) Expression profiling of primary and metastatic ovarian tumors reveals differences indicative of aggressive disease. PLoS One 9:e94476
De Martino, Federico; Zimmermann, Jan; Muckli, Lars et al. (2013) Cortical depth dependent functional responses in humans at 7T: improved specificity with 3D GRASE. PLoS One 8:e60514
De Martino, Federico; Moerel, Michelle; van de Moortele, Pierre-Francois et al. (2013) Spatial organization of frequency preference and selectivity in the human inferior colliculus. Nat Commun 4:1386
Vang, Souriya; Wu, Hsin-Ta; Fischer, Andrew et al. (2013) Identification of ovarian cancer metastatic miRNAs. PLoS One 8:e58226
Chowanadisai, Winyoo; Graham, David M; Keen, Carl L et al. (2013) Neurulation and neurite extension require the zinc transporter ZIP12 (slc39a12). Proc Natl Acad Sci U S A 110:9903-8
Lobikin, Maria; Chernet, Brook; Lobo, Daniel et al. (2012) Resting potential, oncogene-induced tumorigenesis, and metastasis: the bioelectric basis of cancer in vivo. Phys Biol 9:065002

Showing the most recent 10 out of 144 publications