Abstract

In addition to obtaining training in the use of ion-selective probes, preliminary experiments are being conducted to map Ca2+ currents around gravi-sensitive plant cells and organs. As part of a NASA-funded project, Prof. Nina Allen, at NCSU, will soon have equipment for measuring ion fluxes with ion-selective probes to study the role of Ca2+ in regulating gravi-stimulated signal transduction in plants. Ion-selective probes will be used to map changes in Ca2+ (and other specific ion) fluxes in response to gravi-stimulation of gravi-sensitive plant organs (e.g. roots) and cells (apical cell of moss protonemata). It is anticipated that this extracellular approach will be combined with intracellular Ca2+ measurements using Ca2+-reporting dyes. During this visit preliminary experiments are being conducted to map Ca2+ fluxes around root tips of Arabidopsis and around apical protonimata cells of the moss Ceratodon. The study study will then be continued in more depth at the Botany Dept, NCSU The BioCurrents Research Center is the only place to provide adequate training.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001395-19
Application #
6414921
Study Section
Project Start
2000-12-01
Project End
2001-11-30
Budget Start
Budget End
Support Year
19
Fiscal Year
2001
Total Cost
$211,312
Indirect Cost

  Institution

Name
Marine Biological Laboratory
Department
Type
DUNS #
001933779
City
Woods Hole
State
MA
Country
United States
Zip Code
02543

  Publications

Demidenko, Eugene; Glaholt, S P; Kyker-Snowman, E et al. (2017) Single toxin dose-response models revisited. Toxicol Appl Pharmacol 314:12-23
Chowanadisai, Winyoo; Messerli, Shanta M; Miller, Daniel H et al. (2016) Cisplatin Resistant Spheroids Model Clinically Relevant Survival Mechanisms in Ovarian Tumors. PLoS One 11:e0151089
De Martino, Federico; Moerel, Michelle; Ugurbil, Kamil et al. (2015) Less noise, more activation: Multiband acquisition schemes for auditory functional MRI. Magn Reson Med 74:462-7
Van Mooy, Benjamin A S; Hmelo, Laura R; Fredricks, Helen F et al. (2014) Quantitative exploration of the contribution of settlement, growth, dispersal and grazing to the accumulation of natural marine biofilms on antifouling and fouling-release coatings. Biofouling 30:223-36
Brodsky, Alexander S; Fischer, Andrew; Miller, Daniel H et al. (2014) Expression profiling of primary and metastatic ovarian tumors reveals differences indicative of aggressive disease. PLoS One 9:e94476
De Martino, Federico; Zimmermann, Jan; Muckli, Lars et al. (2013) Cortical depth dependent functional responses in humans at 7T: improved specificity with 3D GRASE. PLoS One 8:e60514
De Martino, Federico; Moerel, Michelle; van de Moortele, Pierre-Francois et al. (2013) Spatial organization of frequency preference and selectivity in the human inferior colliculus. Nat Commun 4:1386
Vang, Souriya; Wu, Hsin-Ta; Fischer, Andrew et al. (2013) Identification of ovarian cancer metastatic miRNAs. PLoS One 8:e58226
Chowanadisai, Winyoo; Graham, David M; Keen, Carl L et al. (2013) Neurulation and neurite extension require the zinc transporter ZIP12 (slc39a12). Proc Natl Acad Sci U S A 110:9903-8
Graham, David M; Messerli, Mark A; Pethig, Ronald (2012) Spatial manipulation of cells and organelles using single electrode dielectrophoresis. Biotechniques 52:39-43

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