This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cellular expansion is an absolute necessity during the growth and development of plants and fungi. Expansion relies upon the accumulation of inorganic ions, the resulting water influx creates the hydrostatic pressure (turgor) that causes the cell to expand. Cellular expansion is normally asymmetric. Rather than expanding in all directions, there are localized regions of expansion that result in the well-defined final shape of the cell. The extreme example of anisomorphic cell expansion is tip growth. Here, the machinery of expansion is highly concentrated in a small region such that the cell exhibits tubular growth. How does the cell maintain the turgor that drives expansion? How is expansion controlled spatially? We are documenting the role of signal transduction pathways in turgor recovery in the model organism Neurospora crassa. We showed that an osmotic MAP kinase cascade activates ion transport after hyperosmotic treatment, causing rapid recovery of the normal high hydrostatic pressure (turgor)(Eukaryotic Cell 5:460-487, 2006). At the BioCurrents Research Center, I examined a novel signal transduction pathway, identified by osmosensitivity, that includes the CUT gene (a phosphatase). We demonstrated hyperosmotic-induced changes in the ion fluxes in the cut mutant: Ion uptake that was responsible for turgor recovery (glycerol accumulation, also mediated by the MAP kinase cascade, is absent in the cut mutant).

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001395-27
Application #
7953830
Study Section
Special Emphasis Panel (ZRG1-BPC-H (40))
Project Start
2008-12-01
Project End
2009-11-30
Budget Start
2008-12-01
Budget End
2009-11-30
Support Year
27
Fiscal Year
2009
Total Cost
$11,195
Indirect Cost
Name
Marine Biological Laboratory
Department
Type
DUNS #
001933779
City
Woods Hole
State
MA
Country
United States
Zip Code
02543
Demidenko, Eugene; Glaholt, S P; Kyker-Snowman, E et al. (2017) Single toxin dose-response models revisited. Toxicol Appl Pharmacol 314:12-23
Chowanadisai, Winyoo; Messerli, Shanta M; Miller, Daniel H et al. (2016) Cisplatin Resistant Spheroids Model Clinically Relevant Survival Mechanisms in Ovarian Tumors. PLoS One 11:e0151089
De Martino, Federico; Moerel, Michelle; Ugurbil, Kamil et al. (2015) Less noise, more activation: Multiband acquisition schemes for auditory functional MRI. Magn Reson Med 74:462-7
Van Mooy, Benjamin A S; Hmelo, Laura R; Fredricks, Helen F et al. (2014) Quantitative exploration of the contribution of settlement, growth, dispersal and grazing to the accumulation of natural marine biofilms on antifouling and fouling-release coatings. Biofouling 30:223-36
Brodsky, Alexander S; Fischer, Andrew; Miller, Daniel H et al. (2014) Expression profiling of primary and metastatic ovarian tumors reveals differences indicative of aggressive disease. PLoS One 9:e94476
De Martino, Federico; Zimmermann, Jan; Muckli, Lars et al. (2013) Cortical depth dependent functional responses in humans at 7T: improved specificity with 3D GRASE. PLoS One 8:e60514
De Martino, Federico; Moerel, Michelle; van de Moortele, Pierre-Francois et al. (2013) Spatial organization of frequency preference and selectivity in the human inferior colliculus. Nat Commun 4:1386
Vang, Souriya; Wu, Hsin-Ta; Fischer, Andrew et al. (2013) Identification of ovarian cancer metastatic miRNAs. PLoS One 8:e58226
Chowanadisai, Winyoo; Graham, David M; Keen, Carl L et al. (2013) Neurulation and neurite extension require the zinc transporter ZIP12 (slc39a12). Proc Natl Acad Sci U S A 110:9903-8
Graham, David M; Messerli, Mark A; Pethig, Ronald (2012) Spatial manipulation of cells and organelles using single electrode dielectrophoresis. Biotechniques 52:39-43

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