our lab is interested in studying conformational changes in the molecular motors, actin-myosine and microtubules microtubules binding proteins ncd and kinesin. One way of studying these changes is by using spin probes (paramagnetic probes) that bind at specific sites of the protein and look for conformational changes during force generation using EPR spectroscopy. For this reason we have synthesized several nitroxide spin probes. These are ATP analogs, silfhydryl reagent peptides and other compounds. We have also synthesized heavy metal ion derivatives to hit specific targets on proteins for x-ray crystalographic studies. We used the mass spectrometry facility for the identification of these compounds and the intermediates leading to their formation.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001614-18
Application #
6120241
Study Section
Project Start
1999-03-01
Project End
2000-02-29
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
18
Fiscal Year
1999
Total Cost
Indirect Cost
Name
University of California San Francisco
Department
Type
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143
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