Chem Composition of Subchondral Bone in Osteoporosis W/ Infrared Microscopy (1)

Chance, Mark R.

Abstract

Beamline U2B at the National Synchrotron Light Source is being designed and built as an infrared beamline dedicated to the study of biological problems. During the winter shutdown of 1996, the horizontal acceptance of the U2B beamline was increased from 10 to 45 milliradians, in order to increase the overall flux of the beamline, especially in the far infrared. A wedged, CVD diamond window has been installed to separate the UHV vacuum of the VUV ring and the rough vacuum of the beamline. The rough-vacuum mirror box, which will accept the infrared light out of the VUV ring, has been installed. Design of the remainder of the beamline components has been approved and parts are on order. The infrared spectrometer has been purchased, Hinstalled, and is running with an internal infrared source until Hbeamline construction is complete. The spectrometer is a Nicolet HMagna 560, equipped with interchangeable (1) KBr and silicon beamsplitters and (2) MCT and DTGS detectors. These beamsplitter/detector combinations permit data collection in the mid-H(650-4000 cm-1) and far- (10-650 cm-1) infrared regions. Plans are Hunderway to modify this spectrometer for in-vacuum operation and step-scan capabilities. A NicPlan infrared microscope, equipped with a video imaging camera and motorized x-y sample stage, is included in the Nicolet system for infrared microspectroscopy. Finally, 300 ft2 of laboratory space has been awarded to the biological infrared program for sample preparation and characterization.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Center for Research Resources (NCRR)
• Type: Biotechnology Resource Grants (P41)
• Project #: 5P41RR001633-19
• Application #: 6491464
• Project Start: 2001-09-01
• Project End: 2002-08-31
• Support Year: 19
• Fiscal Year: 2001

Institution

• Name: Albert Einstein College of Medicine
• DUNS #: 009095365
• City: Bronx
• State: NY
• Country: United States
• Zip Code: 10461

Publications

Vongsvivut, Jitraporn; Fernandez, Jason; Ekgasit, Sanong et al. (2004) Characterization of supported cylinder-planar germanium waveguide sensors with synchrotron infrared radiation. Appl Spectrosc 58:143-51

Masip, Lluis; Pan, Jonathan L; Haldar, Suranjana et al. (2004) An engineered pathway for the formation of protein disulfide bonds. Science 303:1185-9

Taylor, Colleen M; Watton, Stephen P; Bryngelson, Peter A et al. (2003) Inner-sphere complexation of cobalt(II) 2,9-dimethyl-1,10-phenanthroline ([Co(neo)]2+) with commercial and sol-gel derived silica gel surfaces. Inorg Chem 42:312-20

Huang, Raymond Y; Miller, Lisa M; Carlson, Cathy S et al. (2003) In situ chemistry of osteoporosis revealed by synchrotron infrared microspectroscopy. Bone 33:514-21

Rashidzadeh, Hassan; Khrapunov, Sergei; Chance, Mark R et al. (2003) Solution structure and interdomain interactions of the Saccharomyces cerevisiae ""TATA binding protein"" (TBP) probed by radiolytic protein footprinting. Biochemistry 42:3655-65

Uchida, Takeshi; Takamoto, Keiji; He, Qin et al. (2003) Multiple monovalent ion-dependent pathways for the folding of the L-21 Tetrahymena thermophila ribozyme. J Mol Biol 328:463-78

Dewan, John C; Feeling-Taylor, Angela; Puius, Yoram A et al. (2002) Structure of mutant human carbonmonoxyhemoglobin C (betaE6K) at 2.0 A resolution. Acta Crystallogr D Biol Crystallogr 58:2038-42

Kiselar, J G; Maleknia, S D; Sullivan, M et al. (2002) Hydroxyl radical probe of protein surfaces using synchrotron X-ray radiolysis and mass spectrometry. Int J Radiat Biol 78:101-14

Swisher, Jennifer F; Su, Linhui J; Brenowitz, Michael et al. (2002) Productive folding to the native state by a group II intron ribozyme. J Mol Biol 315:297-310

Dhavan, Gauri M; Crothers, Donald M; Chance, Mark R et al. (2002) Concerted binding and bending of DNA by Escherichia coli integration host factor. J Mol Biol 315:1027-37