Abstract

For the most part, our laboratory has evaluated the effect of hemodilution and hemodynamic manipulation, and nitric oxide manipulation on cerebral ischemia. We accomplish our studies using three different models of cerebral ischemia in the rat. The first model is an open craniotomy model in which the middle cerebral artery is tied off for 120- minutes and allowed to reperfuse for 180-minutes. By in large we have replaced this model with a less invasive model in which a suture is passed (intraluminally) into the middel cerebral artery via the external carotid artery. A final model of cerebral ischemia which we employ evaluates vasospastic cerebral ischemia. In this model a subarachnoid hemorrhage is induced and areas of cerebral ischemia are assessed. During the past year we have assessed the effect of a molecular hemoglobin solution of cerebral ischemia in models of middle cerebral artery occlusion and subarachnoid hemorrahage. We have used the Drexel system to derermine cerebral blood flow, areas of low cerebral blood flow ( ischemia); and we have determined the cerebral infarct volume utilizing the above system.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001638-11
Application #
5223504
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
11
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Ivey, Richard G; Subramanian, Oby; Lorentzen, Travis D et al. (2009) Antibody-based screen for ionizing radiation-dependent changes in the Mammalian proteome for use in biodosimetry. Radiat Res 171:549-61
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Komisaruk, B R; Rosenblatt, J S; Barona, M L et al. (2000) Combined c-fos and 14C-2-deoxyglucose method to differentiate site-specific excitation from disinhibition: analysis of maternal behavior in the rat. Brain Res 859:262-72
Del Cerro, M C (1998) Role of the vomeronasal input in maternal behavior. Psychoneuroendocrinology 23:905-26
Cohen, F S; Yang, Z; Huang, Z et al. (1998) Automatic matching of homologous histological sections. IEEE Trans Biomed Eng 45:642-9
Ali, W S; Cohen, F S (1998) Registering coronal histological 2-D sections of a rat brain with coronal sections of a 3-D brain atlas using geometric curve invariants and B-spline representation. IEEE Trans Med Imaging 17:957-66
Shumsky, J S; Wu, Y; Murphy, E H et al. (1998) Prenatal cocaine exposure does not affect selected GABAA receptor subunit mRNA expression in rabbit visual cortex. Ann N Y Acad Sci 846:371-4
Libon, D J; Bogdanoff, B; Cloud, B S et al. (1998) Declarative and procedural learning, quantitative measures of the hippocampus, and subcortical white alterations in Alzheimer's disease and ischaemic vascular dementia. J Clin Exp Neuropsychol 20:30-41

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