Two projects involving structure determinations of proteins that are drug design targets are described. The proteins are the microsomal triglyceride transfer protein (MTP), and human cytomegalovirus (HCMV) protease. Each of these projects involves the use of three-dimensional structural information to aid in the design of new therapeutic agents. In each case, the goal is to inhibit a novel protein target. Each of these structures projects presents special challenges that require the use of a high brilliance synchrotron radiation source for data collection. In the case of MTP, limits of resolution of native and derivative data from crystals of this large protein (150 kDa) must be extended to solve the structure. In the case of HCMV protease, where two useful derivatives have been identified, limits of resolution, which are favorable along c*, must be extended in the rest of the sphere of reflection in order to produce an interpretable map.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
3P41RR001646-16S1
Application #
6120532
Study Section
Project Start
1998-09-15
Project End
1999-08-14
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
16
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Cornell University
Department
Type
DUNS #
City
Ithaca
State
NY
Country
United States
Zip Code
14850
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