Inner capsid particles of reovirus and rotavirus. Reovirus cores, particles of about 700 ? diameter, crystallize in space group F432, a=1249 ?. The crystals diffract to 3.5 ? resolution. We have used dual Fuji images plates, mounted in a special cassette on the F1 beamline, to collect data in 0.1 degree oscillation frames. Lower resolution data have been measured using the Quantum 4 detector. We have assembled a relatively complete data set, and we have commenced calculations using cryoEM images to determine initial phases. We have also screened for heavy-atom derivatives. The rotavirus ICPs, similar in size and function, crystallize in space group P212121, a=700 ?, b=1000 ?, c=1400 ?. We have collected a partial high-resolution data set, using dual image plates, as well as a much more complete lower-resolution data set, using the Quantum 4 detector. Human transferrin receptor. We have determined the structure of the transferrin receptor ectodomain to about 3.2 ? resolution, using data collected from unliganded crystals as well as crystals soaked in SmCl3 and in the platinum compound PIP. The space group is P212121, a = 105, b = 217, c = 364 (8 polypeptide chains per asymmetric unit). The Quantum 4 detector on F1 was important for obtaining sufficiently accurate data. The 70 kDa polypeptide chain folds into three domains, one of which mediates dimerization. Another domain bears a striking and unanticipated similarity to certain metalloproteases. The structure is being refined. Clathrin terminal domain. We have determined the structure of the 50 kDa N-terminal domain of clathrin. There is a globular portion, with a 7-blade beta propeller fold, and a stem-like region based on an alpha-helical zig-zag. HIV reverse transcriptase. We have determined the structure of a template/primer/dNTP complex of HIV reverse transcriptase at 3 ? resolution, using data collected at F1 with the Quantum 4 detector. The crystals are in space group P212121, a = 78.5, b = 150.0, c = 279.3. The structure reveals aspects of the catalytic mechanism and suggests explanations for the effects of drug resistance mutations. Papillomavirus L1. We have begun studies of crystals of recombinant pentamers of HPV L1. There are several crystal forms, all with very large unit cells.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001646-17
Application #
6220493
Study Section
Project Start
1999-08-15
Project End
2000-08-14
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
17
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Cornell University
Department
Type
DUNS #
City
Ithaca
State
NY
Country
United States
Zip Code
14850
Kozlov, Guennadi; Wong, Kathy; Gehring, Kalle (2018) Crystal structure of the Legionella effector Lem22. Proteins 86:263-267
Ménade, Marie; Kozlov, Guennadi; Trempe, Jean-François et al. (2018) Structures of ubiquitin-like (Ubl) and Hsp90-like domains of sacsin provide insight into pathological mutations. J Biol Chem 293:12832-12842
Xu, Jie; Kozlov, Guennadi; McPherson, Peter S et al. (2018) A PH-like domain of the Rab12 guanine nucleotide exchange factor DENND3 binds actin and is required for autophagy. J Biol Chem 293:4566-4574
Dean, Dexter N; Rana, Pratip; Campbell, Ryan P et al. (2018) Propagation of an A? Dodecamer Strain Involves a Three-Step Mechanism and a Key Intermediate. Biophys J 114:539-549
Chen, Yu Seby; Kozlov, Guennadi; Fakih, Rayan et al. (2018) The cyclic nucleotide-binding homology domain of the integral membrane protein CNNM mediates dimerization and is required for Mg2+ efflux activity. J Biol Chem 293:19998-20007
Xu, Caishuang; Kozlov, Guennadi; Wong, Kathy et al. (2016) Crystal Structure of the Salmonella Typhimurium Effector GtgE. PLoS One 11:e0166643
Cogliati, Massimo; Zani, Alberto; Rickerts, Volker et al. (2016) Multilocus sequence typing analysis reveals that Cryptococcus neoformans var. neoformans is a recombinant population. Fungal Genet Biol 87:22-9
Oot, Rebecca A; Kane, Patricia M; Berry, Edward A et al. (2016) Crystal structure of yeast V1-ATPase in the autoinhibited state. EMBO J 35:1694-706
Lucido, Michael J; Orlando, Benjamin J; Vecchio, Alex J et al. (2016) Crystal Structure of Aspirin-Acetylated Human Cyclooxygenase-2: Insight into the Formation of Products with Reversed Stereochemistry. Biochemistry 55:1226-38
Bauman, Joseph D; Harrison, Jerry Joe E K; Arnold, Eddy (2016) Rapid experimental SAD phasing and hot-spot identification with halogenated fragments. IUCrJ 3:51-60

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