CHESS F1/A1/F2 beam time was used for high resolution, X-ray diffraction data collection of cocrystalline preparations of eukaryotic transcription factors complexed with DNA. Expression of nuclear genes encoding proteins in eukaryotes is controlled at the level of RNA polymerase II transcription by a complex multisubunit assembly of proteins that interact specifically with each other and with three distinct DNA targets within each gene's promoter or control region. Our ongoing crystallographic measurements are examining three representative examples of transcription factors interacting with each of the DNA targets found in a typical class II promoter. Recent progress includes structure determinations for sterol regulatory element binding protein (SREBP) 1a bound to its DNA target the sterol regulatory element (StRE), and SREBP 2 also bound to the StRE. CHESS F1/F2 beam time was used for high resolution, X-ray diffraction data collection of a cocrystalline preparation of a eukaryotic translation factor complexed with RNA. Expression of nuclear genes encoding proteins in eukaryotes is controlled at the level of ribosomal translation by a complex multisubunit assembly of proteins that interact specifically with each other and with two distinct RNA targets within each gene's mRNA. Our ongoing crystallographic measurements are examining the structure of the cap-binding protein (eIF4E) recognizing the 5 mRNA cap. Recent progress includes structure determinations of complexes of eIF4E bound to a dinucleotide mRNA (7-methylGpppG), and eIF4E bound to both 7-methyl-GDP and 4EBP1 (an inhibitor of translation).

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001646-17
Application #
6220471
Study Section
Project Start
1999-08-15
Project End
2000-08-14
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
17
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Cornell University
Department
Type
DUNS #
City
Ithaca
State
NY
Country
United States
Zip Code
14850
Kozlov, Guennadi; Wong, Kathy; Gehring, Kalle (2018) Crystal structure of the Legionella effector Lem22. Proteins 86:263-267
Ménade, Marie; Kozlov, Guennadi; Trempe, Jean-François et al. (2018) Structures of ubiquitin-like (Ubl) and Hsp90-like domains of sacsin provide insight into pathological mutations. J Biol Chem 293:12832-12842
Xu, Jie; Kozlov, Guennadi; McPherson, Peter S et al. (2018) A PH-like domain of the Rab12 guanine nucleotide exchange factor DENND3 binds actin and is required for autophagy. J Biol Chem 293:4566-4574
Dean, Dexter N; Rana, Pratip; Campbell, Ryan P et al. (2018) Propagation of an A? Dodecamer Strain Involves a Three-Step Mechanism and a Key Intermediate. Biophys J 114:539-549
Chen, Yu Seby; Kozlov, Guennadi; Fakih, Rayan et al. (2018) The cyclic nucleotide-binding homology domain of the integral membrane protein CNNM mediates dimerization and is required for Mg2+ efflux activity. J Biol Chem 293:19998-20007
Xu, Caishuang; Kozlov, Guennadi; Wong, Kathy et al. (2016) Crystal Structure of the Salmonella Typhimurium Effector GtgE. PLoS One 11:e0166643
Cogliati, Massimo; Zani, Alberto; Rickerts, Volker et al. (2016) Multilocus sequence typing analysis reveals that Cryptococcus neoformans var. neoformans is a recombinant population. Fungal Genet Biol 87:22-9
Oot, Rebecca A; Kane, Patricia M; Berry, Edward A et al. (2016) Crystal structure of yeast V1-ATPase in the autoinhibited state. EMBO J 35:1694-706
Lucido, Michael J; Orlando, Benjamin J; Vecchio, Alex J et al. (2016) Crystal Structure of Aspirin-Acetylated Human Cyclooxygenase-2: Insight into the Formation of Products with Reversed Stereochemistry. Biochemistry 55:1226-38
Bauman, Joseph D; Harrison, Jerry Joe E K; Arnold, Eddy (2016) Rapid experimental SAD phasing and hot-spot identification with halogenated fragments. IUCrJ 3:51-60

Showing the most recent 10 out of 375 publications