Abstract

They are interested. in the structure of the ACDS (Acetyl-CoA Decarbonylase SYnd'ase) multienzyme complex which is responsible for the synthesis and cleavage of acetyl-CoA in methanogens. To determine the detailed oligorneric structure of the complex ( -1.6-2 MDA), accurate molecular masses are needed. ACDS complexes from Methanosarcina therinophila and M. barkeri contain five different subunits designated -a, P, y, 6, and F- The complex can also be dissociated into three protein subcomponents, one of which is (a + e). It is thought that the (a+e) particle is a heterotetramer (oc2e2) of approximately 220 kDa, but gel filtration data have indicated a value of 350 kDa. To start this project, fi-eeze-dried specimens of the (a+c:) particle were prepared. Preliminary ma s measurements indicate that it is a heterotetramer. The (y + 8) subcomponent from the complex has also been examined in the STEM for mass analysis, which is being performed now.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001777-18
Application #
6308944
Study Section
Project Start
2000-04-01
Project End
2001-03-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
18
Fiscal Year
2000
Total Cost
$9,722
Indirect Cost

  Institution

Name
Brookhaven National Laboratory
Department
Type
DUNS #
027579460
City
Upton
State
NY
Country
United States
Zip Code
11973

  Publications

Hind, Geoffrey; Wall, Joseph S; Várkonyi, Zsuzsanna et al. (2014) Membrane crystals of plant light-harvesting complex II disassemble reversibly in light. Plant Cell Physiol 55:1296-303
Garofalo, Robert; Mustanski, Brian; Johnson, Amy et al. (2010) Exploring factors that underlie racial/ethnic disparities in HIV risk among young men who have sex with men. J Urban Health 87:318-323
Joshi, V N; Mitra, D; England, M D et al. (2010) Large Covalently Linked Fluorescent and Gold Nanoparticle Immunoprobes. Microsc Microanal 16:966-967
Ackerson, Christopher J; Powell, Richard D; Hainfeld, James F (2010) Site-specific biomolecule labeling with gold clusters. Methods Enzymol 481:195-230
Hu, Minghui; Zhang, Yian-Biao; Qian, Luping et al. (2008) Three-dimensional structure of human chromatin accessibility complex hCHRAC by electron microscopy. J Struct Biol 164:263-9
Hu, Minghui; Qian, Luping; Brinas, Raymond P et al. (2008) Gold nanoparticle-protein arrays improve resolution for cryo-electron microscopy. J Struct Biol 161:83-91
Garofalo, Robert; Mustanski, Brian; Donenberg, Geri (2008) Parents know and parents matter;is it time to develop family-based HIV prevention programs for young men who have sex with men? J Adolesc Health 43:201-4
Brinas, Raymond P; Hu, Minghui; Qian, Luping et al. (2008) Gold nanoparticle size controlled by polymeric Au(I) thiolate precursor size. J Am Chem Soc 130:975-82
Garofalo, Robert; Mustanski, Brian S; McKirnan, David J et al. (2007) Methamphetamine and young men who have sex with men: understanding patterns and correlates of use and the association with HIV-related sexual risk. Arch Pediatr Adolesc Med 161:591-6
Mustanski, Brian; Garofalo, Robert; Herrick, Amy et al. (2007) Psychosocial health problems increase risk for HIV among urban young men who have sex with men: preliminary evidence of a syndemic in need of attention. Ann Behav Med 34:37-45

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