THE SIR SUPPLIED DR. JAMES WITH 1G OF D, L-ARGININE-GUANIDO 13C, 15N2. ARGININE WITH 13C AND 15N LABELED IN THE GUANIDO GROUP IS OBTAINED FROM THE NATIONAL STABLE ISOTOPE RESOURCE AT LOS ALAMOS NATIONAL LABORATORY. THIS LABELED ARGININE IS INCORPORATED INTO A 25 AMINO ACYL PEPTIDE WHICH CONTAINS THE RNA BINDING DOMAIN OF THE TAT PROTEIN FROM HIV-1 AND HAS BEEN SHOWN TO PROMOTE HIV-1 REPLICATION CELLS. WHILE THE 25-MER HAS HAD ITS SOLUTION STRUCTURE DETERMINED VIA HOMONUCLEAR PROTON NMR STUDIES, THE SIDE-CHAIN PROTON RESONANCES OF THE SEVERAL ARGININES CANNOT BE RESOLVED IN THE SPECTRA. PLACING THE LABELED ARGININE AT THE POSITION CORRESPONDING TO R52 IN HIV-1 TAT IS IMPORTANT IS SUBSEQUENT STUDIES OF THE BIOLOGICALLY ACTIVE TAT PEPTIDE WITH TAR, THE RNA TO WHICH IT BINDS IN VIRAL REPLICATION. R52 HAS BEEN DEMONSTRATED TO BE ESSENTIAL FOR TAT ACTIVITY. THE STRUCTURE OF THIS SELECTIVELY LABELED PEPTIDE BOUND TO TAR SHOULD BE MUCH BETTER DEFINED BY USE OF THE LABELED ARG. CONSEQUENTLY, THE STRUCTURE DETERMINED SHOULD PROVIDE A BETTER TARGET FOR DEVELOPMENT OF A DRUG TO COMBAT AIDS.
| Martinez, Rodolfo A; Glass, David R; Ortiz, Erick G et al. (2014) Synthesis of isotopically labeled 1,3-dithiane. J Labelled Comp Radiopharm 57:338-41 |
| Martinez, Rodolfo A; Glass, David R; Ortiz, Erick G et al. (2013) Large-scale preparation of (13) C-labeled 2-(phenylthio)acetic acid and the corresponding labeled sulfoxides and sulfones. J Labelled Comp Radiopharm 56:31-5 |
| Creager, Melinda S; Jenkins, Janelle E; Thagard-Yeaman, Leigh A et al. (2010) Solid-state NMR comparison of various spiders' dragline silk fiber. Biomacromolecules 11:2039-43 |
| Jenkins, Janelle E; Creager, Melinda S; Lewis, Randolph V et al. (2010) Quantitative Correlation between the protein primary sequences and secondary structures in spider dragline silks. Biomacromolecules 11:192-200 |
| Kim, Sun Hee; Aznar, Constantino; Brynda, Marcin et al. (2004) An EPR, ESEEM, structural NMR, and DFT study of a synthetic model for the covalently ring-linked tyrosine-histidine structure in the heme-copper oxidases. J Am Chem Soc 126:2328-38 |
| Ollivault-Shiflett, Morgane; Kimball, David B; Silks, L A Pete (2004) Synthesis of chiral 13C,77Se-labeled selones. J Org Chem 69:5150-2 |
| Schmidt, Bryan; Hillier, Warwick; McCracken, John et al. (2004) The use of stable isotopes and spectroscopy to investigate the energy transducing function of cytochrome c oxidase. Biochim Biophys Acta 1655:248-55 |
| Schmidt, Bryan; McCracken, John; Ferguson-Miller, Shelagh (2003) A discrete water exit pathway in the membrane protein cytochrome c oxidase. Proc Natl Acad Sci U S A 100:15539-42 |
| Gray, Chandele R; Sanz-Cervera, Juan F; Silks, Louis A et al. (2003) Studies on the biosynthesis of asperparaline A: origin of the spirosuccinimde ring system. J Am Chem Soc 125:14692-3 |
| Van Dien, Stephen J; Strovas, Tim; Lidstrom, Mary E (2003) Quantification of central metabolic fluxes in the facultative methylotroph methylobacterium extorquens AM1 using 13C-label tracing and mass spectrometry. Biotechnol Bioeng 84:45-55 |
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