UNIFORMLY 13C-LABELED GLYCEROL IS POTENTIALLY AN IMPORTANT PRECURSOR FOR THE PRODUCTION OF LABELED PROTEINS. THIS YEAR WE FINISHED DEVELOPING A NOVEL SYNTHESIS OF [U-13C3]GLYCEROL FROM 13CO2. IN THIS SYNTHETIC ROUTE, THE THREE CARBON LABELED BACKBONE IS PRODUCED BY ADDING 13CO2 TO LITHIUM [1,2-13C2]ACETYLIDE TO YIELD [U-13C3]PROPIOLIC ACID. THE OXIDATION STATE OF PROPIOLATE IS ADJUSTED TO YIELD [U-13C3]GLYCEROL. THIS METHOD REPRESENTS A SIGNIFICANT IMPROVEMENT OF IN THE LITERATURE SYNTHESIS OF LABELED GLYCEROL AND WAS REPORTED IN THE SIR REPORT (NEWSLETTER) THIS YEAR. IN ADDITION, WE ARE DEVELOPING A NEW ROUTE TO GLYCEROL WHICH WILL ALLOW DIFFERENTIAL LABELING OF C-2 AND C-1,3. MS EMILY STOCKING A GRADUATE FROM THE UNIVERSITY OF CALIFORNIA AT DAVIS IS RECEIVING TRAINING IN THE SYNTHESIS.
| Martinez, Rodolfo A; Glass, David R; Ortiz, Erick G et al. (2014) Synthesis of isotopically labeled 1,3-dithiane. J Labelled Comp Radiopharm 57:338-41 |
| Martinez, Rodolfo A; Glass, David R; Ortiz, Erick G et al. (2013) Large-scale preparation of (13) C-labeled 2-(phenylthio)acetic acid and the corresponding labeled sulfoxides and sulfones. J Labelled Comp Radiopharm 56:31-5 |
| Creager, Melinda S; Jenkins, Janelle E; Thagard-Yeaman, Leigh A et al. (2010) Solid-state NMR comparison of various spiders' dragline silk fiber. Biomacromolecules 11:2039-43 |
| Jenkins, Janelle E; Creager, Melinda S; Lewis, Randolph V et al. (2010) Quantitative Correlation between the protein primary sequences and secondary structures in spider dragline silks. Biomacromolecules 11:192-200 |
| Kim, Sun Hee; Aznar, Constantino; Brynda, Marcin et al. (2004) An EPR, ESEEM, structural NMR, and DFT study of a synthetic model for the covalently ring-linked tyrosine-histidine structure in the heme-copper oxidases. J Am Chem Soc 126:2328-38 |
| Ollivault-Shiflett, Morgane; Kimball, David B; Silks, L A Pete (2004) Synthesis of chiral 13C,77Se-labeled selones. J Org Chem 69:5150-2 |
| Schmidt, Bryan; Hillier, Warwick; McCracken, John et al. (2004) The use of stable isotopes and spectroscopy to investigate the energy transducing function of cytochrome c oxidase. Biochim Biophys Acta 1655:248-55 |
| Schmidt, Bryan; McCracken, John; Ferguson-Miller, Shelagh (2003) A discrete water exit pathway in the membrane protein cytochrome c oxidase. Proc Natl Acad Sci U S A 100:15539-42 |
| Gray, Chandele R; Sanz-Cervera, Juan F; Silks, Louis A et al. (2003) Studies on the biosynthesis of asperparaline A: origin of the spirosuccinimde ring system. J Am Chem Soc 125:14692-3 |
| Van Dien, Stephen J; Strovas, Tim; Lidstrom, Mary E (2003) Quantification of central metabolic fluxes in the facultative methylotroph methylobacterium extorquens AM1 using 13C-label tracing and mass spectrometry. Biotechnol Bioeng 84:45-55 |
Showing the most recent 10 out of 72 publications
