The SIR provided 74% K 15 N03 containing 87.6g of isotope; 803.9g 17.5% K""""""""N03 contining 131g of isotope; 4978.9 g 17.5% K 15 NO containing 10.4g of isotope; 4 lOg 21% of K 15 N63 continaing 186g of isotope; 2030. 1 g Improved understanding of the nitrogen cycle in estuaries is needed to help explain how anthropogenic changes in nitrogen inputs are affecting coastal systems. We propose to experimentally test conceptual and numerical models of nitrogen dynamics in an estuary by adding 15N-enriched dissolved inorganic nitrogen to the upper reaches of the Parker River-Plum Island Sound estuary, the site of an ongoing NSF-funded Land Margin Ecosystem Research (LMER) program. Previous whole-system labeling experiments in forested watersheds, lakes and rivers have demonstrated the feasibility, power and cost effectiveness of the tracer addition approach. In the Parker River-Plum Island Sound estuary, intensive measurements of the distribution of the added nitrogen tracer will yield estimates of fluxes and residence times of nitrogen in the water, food web components and sediments. The 15N addition experiment will provide information to test and refine complex models of estuarine N flow that are otherwise very difficult to validate. The product will be greatly improved understanding of the transport, transformation and fate of nitrogen in riverine saltmarsh estuaries.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR002231-14
Application #
6281462
Study Section
Project Start
1998-01-15
Project End
1999-01-14
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
14
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Los Alamos National Lab
Department
Type
DUNS #
City
Los Alamos
State
NM
Country
United States
Zip Code
87545
Martinez, Rodolfo A; Glass, David R; Ortiz, Erick G et al. (2014) Synthesis of isotopically labeled 1,3-dithiane. J Labelled Comp Radiopharm 57:338-41
Martinez, Rodolfo A; Glass, David R; Ortiz, Erick G et al. (2013) Large-scale preparation of (13) C-labeled 2-(phenylthio)acetic acid and the corresponding labeled sulfoxides and sulfones. J Labelled Comp Radiopharm 56:31-5
Jenkins, Janelle E; Creager, Melinda S; Lewis, Randolph V et al. (2010) Quantitative Correlation between the protein primary sequences and secondary structures in spider dragline silks. Biomacromolecules 11:192-200
Creager, Melinda S; Jenkins, Janelle E; Thagard-Yeaman, Leigh A et al. (2010) Solid-state NMR comparison of various spiders' dragline silk fiber. Biomacromolecules 11:2039-43
Kim, Sun Hee; Aznar, Constantino; Brynda, Marcin et al. (2004) An EPR, ESEEM, structural NMR, and DFT study of a synthetic model for the covalently ring-linked tyrosine-histidine structure in the heme-copper oxidases. J Am Chem Soc 126:2328-38
Ollivault-Shiflett, Morgane; Kimball, David B; Silks, L A Pete (2004) Synthesis of chiral 13C,77Se-labeled selones. J Org Chem 69:5150-2
Schmidt, Bryan; Hillier, Warwick; McCracken, John et al. (2004) The use of stable isotopes and spectroscopy to investigate the energy transducing function of cytochrome c oxidase. Biochim Biophys Acta 1655:248-55
Schmidt, Bryan; McCracken, John; Ferguson-Miller, Shelagh (2003) A discrete water exit pathway in the membrane protein cytochrome c oxidase. Proc Natl Acad Sci U S A 100:15539-42
Gray, Chandele R; Sanz-Cervera, Juan F; Silks, Louis A et al. (2003) Studies on the biosynthesis of asperparaline A: origin of the spirosuccinimde ring system. J Am Chem Soc 125:14692-3
Van Dien, Stephen J; Strovas, Tim; Lidstrom, Mary E (2003) Quantification of central metabolic fluxes in the facultative methylotroph methylobacterium extorquens AM1 using 13C-label tracing and mass spectrometry. Biotechnol Bioeng 84:45-55

Showing the most recent 10 out of 72 publications