The SIR provided [U-""""""""C]Glucal While the role of carbohydrates is wall recognized in a variety of important biological phenomena, study of them has been restricted by availability of material. Recent advances in carbohydrate chemistry, including development of solid phase synthesis methods have helped address this limitation, offering general protocols for preparing significant quantities of well defined pure material. Such approaches have already bom fruit in the preparation of members of the Lewis blood group carbohydrates. Expression and recognition of these carbohydrates have been associated with cellular recognition, bacterial infection and cancer, making these interesing targets for conformational analysis to understand their biological roles. Investigations of solution conformation of the Lewis x determinant have been undertaken using proton NMR and heteronuclear NMR at natural abundance. In one instance, the C l' of one of the sugars in tLewis x oligosaccharide was labeled with 13C, providing advantages for conformational analysis. In gerneral experimental data for conformational analysis has been insuffcient to fully define the structure and analysis has relied heavily on modeling.
The aim of preparing the labeled compounds proposed here is to overcome these limitations byrnaking powerful heteronuclear NMR techniques availabe. An important aspect of the novel synthetic methods being developed is that they can be combined effectively with isotopic labeling. This will dramatically improve the NMR results and enhance the conformational analysis of these spedies. The impact of isotopic labeling on the study of RNA illustrates the kind of advantages that can be gained in studying sugar moieties.
| Martinez, Rodolfo A; Glass, David R; Ortiz, Erick G et al. (2014) Synthesis of isotopically labeled 1,3-dithiane. J Labelled Comp Radiopharm 57:338-41 |
| Martinez, Rodolfo A; Glass, David R; Ortiz, Erick G et al. (2013) Large-scale preparation of (13) C-labeled 2-(phenylthio)acetic acid and the corresponding labeled sulfoxides and sulfones. J Labelled Comp Radiopharm 56:31-5 |
| Creager, Melinda S; Jenkins, Janelle E; Thagard-Yeaman, Leigh A et al. (2010) Solid-state NMR comparison of various spiders' dragline silk fiber. Biomacromolecules 11:2039-43 |
| Jenkins, Janelle E; Creager, Melinda S; Lewis, Randolph V et al. (2010) Quantitative Correlation between the protein primary sequences and secondary structures in spider dragline silks. Biomacromolecules 11:192-200 |
| Kim, Sun Hee; Aznar, Constantino; Brynda, Marcin et al. (2004) An EPR, ESEEM, structural NMR, and DFT study of a synthetic model for the covalently ring-linked tyrosine-histidine structure in the heme-copper oxidases. J Am Chem Soc 126:2328-38 |
| Ollivault-Shiflett, Morgane; Kimball, David B; Silks, L A Pete (2004) Synthesis of chiral 13C,77Se-labeled selones. J Org Chem 69:5150-2 |
| Schmidt, Bryan; Hillier, Warwick; McCracken, John et al. (2004) The use of stable isotopes and spectroscopy to investigate the energy transducing function of cytochrome c oxidase. Biochim Biophys Acta 1655:248-55 |
| Schmidt, Bryan; McCracken, John; Ferguson-Miller, Shelagh (2003) A discrete water exit pathway in the membrane protein cytochrome c oxidase. Proc Natl Acad Sci U S A 100:15539-42 |
| Gray, Chandele R; Sanz-Cervera, Juan F; Silks, Louis A et al. (2003) Studies on the biosynthesis of asperparaline A: origin of the spirosuccinimde ring system. J Am Chem Soc 125:14692-3 |
| Van Dien, Stephen J; Strovas, Tim; Lidstrom, Mary E (2003) Quantification of central metabolic fluxes in the facultative methylotroph methylobacterium extorquens AM1 using 13C-label tracing and mass spectrometry. Biotechnol Bioeng 84:45-55 |
Showing the most recent 10 out of 72 publications
