The thick filament is an organized permanent assemblage of myosin molecules in muscles. Vertebrate thick filaments contain C protein, titin, and M band proteins that associate with myosin whereas non-vertebrate filaments contain the rodlike protein paramyosin in the filament core as well as the associated twitchin/projectin homologues of titin/C protein. Because of extensive developmental, genetic, molecular and cellular and biochemical studies, the thick filaments of Caenorhabditis elegans are proposed as a non-vertebrate model for this important macromolecular assembly. A preliminary negative stain reconstruction of the core assembly shows rod-like features surrounding, and cross-linked by, a central tube containing other globular density, perhaps representing other proteins known to associate with the core. Both the core and intact thick filaments are being investigated by cryomicroscopy. In a study of the cores published this year, we have proposed a model for the core and its associated internal proteins. The model fits best with the observed calculated Fourier transform when the paramyosin is arranged as 7 strands around the outside of the core, and the internal proteins are distributed across the gap of single-stranded paramyosin in an asymmetric fashion. Furthermore, the diffraction pattern is sensitive to the fraction of the length occupied by these internal cores, with 25% of the length yielding the best fit.
Bucero, Marta Abril; Bajaj, Chandrajit; Mourrain, Bernard (2016) On the construction of general cubature formula by flat extensions. Linear Algebra Appl 502:104-125 |
Ebeida, Mohamed S; Rushdi, Ahmad A; Awad, Muhammad A et al. (2016) Disk Density Tuning of a Maximal Random Packing. Comput Graph Forum 35:259-269 |
Wensel, Theodore G; Zhang, Zhixian; Anastassov, Ivan A et al. (2016) Structural and molecular bases of rod photoreceptor morphogenesis and disease. Prog Retin Eye Res 55:32-51 |
Baker, Mariah R; Fan, Guizhen; Serysheva, Irina I (2015) Single-Particle Cryo-EM of the Ryanodine Receptor Channel in an Aqueous Environment. Eur J Transl Myol 25:4803 |
Rushdi, Ahmad A; Mitchell, Scott A; Bajaj, Chandrajit L et al. (2015) Robust All-quad Meshing of Domains with Connected Regions. Procedia Eng 124:96-108 |
Edwards, John; Daniel, Eric; Pascucci, Valerio et al. (2015) Approximating the Generalized Voronoi Diagram of Closely Spaced Objects. Comput Graph Forum 34:299-309 |
Wensel, Theodore G; Gilliam, Jared C (2015) Three-dimensional architecture of murine rod cilium revealed by cryo-EM. Methods Mol Biol 1271:267-92 |
Jeter, Cameron B; Patel, Saumil S; Morris, Jeffrey S et al. (2015) Oculomotor executive function abnormalities with increased tic severity in Tourette syndrome. J Child Psychol Psychiatry 56:193-202 |
Zhang, Qin; Cha, Deukhyun; Bajaj, Chandrajit (2015) Quality Partitioned Meshing of Multi-Material Objects. Procedia Eng 124:187-199 |
Baker, Mariah R; Fan, Guizhen; Serysheva, Irina I (2015) Single-particle cryo-EM of the ryanodine receptor channel in an aqueous environment. Eur J Transl Myol 25:35-48 |
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