After many years of effort in solving the molecular structure of crotoxin complex crystal, we have concluded that the crystal has variable motifs which make it impractical for structural determination by classical electron crystallographic procedures. In order to obtain independent confirmation that the unit cell motifs vary across the crystals, we have initiated an effort to study this specimen in an atomic force microscope. This technology is well advanced in the laboratory of Professor A. Engel. We hope to evaluate if this approach will provide a useful indication of the suitability of thin protein crystals for electron crystallographic analysis.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR002250-14
Application #
6120877
Study Section
Project Start
1998-12-01
Project End
1999-11-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
14
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Baylor College of Medicine
Department
Type
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030
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