The National Center for Macromolecular Imaging (NCMI) has been supported by NCRR since 1985. We focus on advancing electron cryomicroscopy for structural biology towards atomic resolution. This period, we determined the structures of 7 viruses and molecular machines at 6.5-9.5 A. Most secondary structure elements of the proteins can be seen. This resulted from innovation in instrument set-up, data collection, refinement, feature extraction and visualization. We published 72 manuscripts related to core, collaboration, service, training and dissemination, and sponsored 11 workshops and symposia. In the coming period, we aim 1) to extend the resolution of single particle reconstructions towards 3-4 A; and 2) to transform our experimental and computational steps so that biologists can quickly obtain structures at sub-nanometer resolution. We will install a new cryomicroscope, upgrade instruments, and improve the quality and efficiency of specimen preparation, data collection, pre-processing, refinement, structure analysis, data mining, deposition, visualization, animation and data management. Our staff will partner in some cases with instrument and software developers at other centers and industry. This core development is motivated by 13 collaborative and 16 service projects. These span the spectrum of specimens, including subcellular ordered assemblies, viruses, ion channels, receptors, sensors, molecular machines, multi-subunit enzymes and nano-particles with sizes from ~300 kDa to 200 MDa. We will disseminate our technology via publications, workshops, symposia, a book on cryo-EM and video conferencing. We will adopt an open-source code policy for our software. We anticipate a continuous growth in demand for our facility from global users and will emulate the management model of a synchrotron beamline. A committee of prominent scientists will annually advise us. NCMI will lead in integrating all aspects of cryo-EM structure determination to ? ?
| Ebeida, Mohamed S; Rushdi, Ahmad A; Awad, Muhammad A et al. (2016) Disk Density Tuning of a Maximal Random Packing. Comput Graph Forum 35:259-269 |
| Wensel, Theodore G; Zhang, Zhixian; Anastassov, Ivan A et al. (2016) Structural and molecular bases of rod photoreceptor morphogenesis and disease. Prog Retin Eye Res 55:32-51 |
| Bucero, Marta Abril; Bajaj, Chandrajit; Mourrain, Bernard (2016) On the construction of general cubature formula by flat extensions. Linear Algebra Appl 502:104-125 |
| Baker, Mariah R; Fan, Guizhen; Serysheva, Irina I (2015) Single-Particle Cryo-EM of the Ryanodine Receptor Channel in an Aqueous Environment. Eur J Transl Myol 25:4803 |
| Rushdi, Ahmad A; Mitchell, Scott A; Bajaj, Chandrajit L et al. (2015) Robust All-quad Meshing of Domains with Connected Regions. Procedia Eng 124:96-108 |
| Edwards, John; Daniel, Eric; Pascucci, Valerio et al. (2015) Approximating the Generalized Voronoi Diagram of Closely Spaced Objects. Comput Graph Forum 34:299-309 |
| Wensel, Theodore G; Gilliam, Jared C (2015) Three-dimensional architecture of murine rod cilium revealed by cryo-EM. Methods Mol Biol 1271:267-92 |
| Jeter, Cameron B; Patel, Saumil S; Morris, Jeffrey S et al. (2015) Oculomotor executive function abnormalities with increased tic severity in Tourette syndrome. J Child Psychol Psychiatry 56:193-202 |
| Zhang, Qin; Cha, Deukhyun; Bajaj, Chandrajit (2015) Quality Partitioned Meshing of Multi-Material Objects. Procedia Eng 124:187-199 |
| Baker, Mariah R; Fan, Guizhen; Serysheva, Irina I (2015) Single-particle cryo-EM of the ryanodine receptor channel in an aqueous environment. Eur J Transl Myol 25:35-48 |
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