In order to understand the mechanism of the interaction between chymotrypsin and its inhibitor - OMTKY3, it is necessary to relate the biochemical behavior of the mutants of OMTKY3 to their structural properties. pH titration by NMR can provide such information. By means of pH titration, we can obtain the pKa value of different amino acid residues. By comparison of these pKa values,, those of wild type and those of other mutants, we can obtain important structural information for OMTKY3 and arrive at a further understanding of its inhibitory action on chymotrypsin.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
2P41RR002301-16
Application #
6309108
Study Section
Project Start
2000-04-15
Project End
2005-02-28
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
16
Fiscal Year
2000
Total Cost
$7,533
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
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