Abstract

The goal of this study is to identify the binding sites for antibodies injected into early chick embryos during the initial stages of formation of the eye, specifically the optic cup. The project utilizes the confocal microscope in the Resource. Localization of antibodies against laminin, anti-N-CAM injected into the otic region, and anti-N-cadherin injected beneath the neural plate are under study. Each antibody has a specific inhibitory effect on development of the organ and the goal is to identify the mode of action by examining the site where the injected antibody binds. 3D data sets of optical sections have been obtained in each case, and 3D reconstructions are being made. This work will continue in the coming year. 8-31-96

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR002483-10
Application #
5224056
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
10
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Deng, Manqi; Williams, Carmen J; Schultz, Richard M (2005) Role of MAP kinase and myosin light chain kinase in chromosome-induced development of mouse egg polarity. Dev Biol 278:358-66
Tutuncu, Levent; Stein, Paula; Ord, Teri S et al. (2004) Calreticulin on the mouse egg surface mediates transmembrane signaling linked to cell cycle resumption. Dev Biol 270:246-60
Brown, Rebecca L; August, Shelley L; Williams, Carmen J et al. (2003) AKAP7gamma is a nuclear RI-binding AKAP. Biochem Biophys Res Commun 306:394-401
Deng, Manqi; Kishikawa, Hidefumi; Yanagimachi, Ryuzo et al. (2003) Chromatin-mediated cortical granule redistribution is responsible for the formation of the cortical granule-free domain in mouse eggs. Dev Biol 257:166-76
Brown, Rebecca L; Ord, Teri; Moss, Stuart B et al. (2002) A-kinase anchor proteins as potential regulators of protein kinase A function in oocytes. Biol Reprod 67:981-7
Li, J; Zhu, X; Ashton, F T et al. (2001) Sensory neuroanatomy of a passively ingested nematode parasite, Haemonchus contortus: amphidial neurons of the third-stage larva. J Parasitol 87:65-72
Robson, P; Stein, P; Zhou, B et al. (2001) Inner cell mass-specific expression of a cell adhesion molecule (PECAM-1/CD31) in the mouse blastocyst. Dev Biol 234:317-29
Travis, A J; Merdiushev, T; Vargas, L A et al. (2001) Expression and localization of caveolin-1, and the presence of membrane rafts, in mouse and Guinea pig spermatozoa. Dev Biol 240:599-610
Stein, P; Schultz, R M (2000) Initiation of a chromatin-based transcriptionally repressive state in the preimplantation mouse embryo: lack of a primary role for expression of somatic histone H1. Mol Reprod Dev 55:241-8
Li, J; Ashton, F T; Gamble, H R et al. (2000) Sensory neuroanatomy of a passively ingested nematode parasite, Haemonchus contortus: amphidial neurons of the first stage larva. J Comp Neurol 417:299-314

Showing the most recent 10 out of 38 publications