Abstract

We have explored the use of green fluorescent protein as a tracer dye for cell movements in developing early zebrafish (Danio rerio) embryos. We study the expression of the homeodomain transcription factor zOtx1 to study the movement of cells that ectopically express this factor. Embryos injected with RNA encoding zOtx1 appear to have gastrulation defects indicative of abnormal cell movements. Co-injection of GFP and zOtx1 RNAs into single cells at the 16 cell stage yields bright fluorescence that can be detected in a subpopulation (about 5-10%) of cells in the 5 hour blastula. We will study the lineage and movement of these labeled cells in order to elucidate the possible role of zOtx1 in restriction of cell movements and promotion of cell adhesion. 8-31-96

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR002483-10S1
Application #
2556275
Study Section
Project Start
Project End
Budget Start
1996-10-01
Budget End
1997-09-30
Support Year
10
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Deng, Manqi; Williams, Carmen J; Schultz, Richard M (2005) Role of MAP kinase and myosin light chain kinase in chromosome-induced development of mouse egg polarity. Dev Biol 278:358-66
Tutuncu, Levent; Stein, Paula; Ord, Teri S et al. (2004) Calreticulin on the mouse egg surface mediates transmembrane signaling linked to cell cycle resumption. Dev Biol 270:246-60
Brown, Rebecca L; August, Shelley L; Williams, Carmen J et al. (2003) AKAP7gamma is a nuclear RI-binding AKAP. Biochem Biophys Res Commun 306:394-401
Deng, Manqi; Kishikawa, Hidefumi; Yanagimachi, Ryuzo et al. (2003) Chromatin-mediated cortical granule redistribution is responsible for the formation of the cortical granule-free domain in mouse eggs. Dev Biol 257:166-76
Brown, Rebecca L; Ord, Teri; Moss, Stuart B et al. (2002) A-kinase anchor proteins as potential regulators of protein kinase A function in oocytes. Biol Reprod 67:981-7
Li, J; Zhu, X; Ashton, F T et al. (2001) Sensory neuroanatomy of a passively ingested nematode parasite, Haemonchus contortus: amphidial neurons of the third-stage larva. J Parasitol 87:65-72
Robson, P; Stein, P; Zhou, B et al. (2001) Inner cell mass-specific expression of a cell adhesion molecule (PECAM-1/CD31) in the mouse blastocyst. Dev Biol 234:317-29
Travis, A J; Merdiushev, T; Vargas, L A et al. (2001) Expression and localization of caveolin-1, and the presence of membrane rafts, in mouse and Guinea pig spermatozoa. Dev Biol 240:599-610
Stein, P; Schultz, R M (2000) Initiation of a chromatin-based transcriptionally repressive state in the preimplantation mouse embryo: lack of a primary role for expression of somatic histone H1. Mol Reprod Dev 55:241-8
Li, J; Ashton, F T; Gamble, H R et al. (2000) Sensory neuroanatomy of a passively ingested nematode parasite, Haemonchus contortus: amphidial neurons of the first stage larva. J Comp Neurol 417:299-314

Showing the most recent 10 out of 38 publications