Abstract

We have designed and used a versatile UV resonance Raman spectrometer for quantitative analysis of cancerous in human colon tissues. The goal is to identify and quantify various chemicals in normal and diseased tissues and correlate with pathology. We have examined normal, adenomatous polyps and adenocarcinoma samples from six patients using 251 nm laser excitation. Spectra exhibit marked differences and we attribute these to increased nucleic acid to protein content in precancer and cancerous tissues compared to normal tissues. We are currently verifying these results by studying using an another laser excitation at 240 nm and using microspectroscopy.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR002594-13
Application #
6281777
Study Section
Project Start
1998-06-01
Project End
1999-05-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
13
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Massachusetts Institute of Technology
Department
Type
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02139

  Publications

Shih, Wei-Chuan; Bechtel, Kate L; Rebec, Mihailo V (2015) Noninvasive glucose sensing by transcutaneous Raman spectroscopy. J Biomed Opt 20:051036
Dudzik, Jonathan; Chang, Wen-Chi; Kannan, A M et al. (2013) Cross-linked glucose oxidase clusters for biofuel cell anode catalysts. Biofabrication 5:035009
Sathyavathi, R; Dingari, Narahara Chari; Barman, Ishan et al. (2013) Raman spectroscopy provides a powerful, rapid diagnostic tool for the detection of tuberculous meningitis in ex vivo cerebrospinal fluid samples. J Biophotonics 6:567-72
Dingari, Narahara Chari; Barman, Ishan; Saha, Anushree et al. (2013) Development and comparative assessment of Raman spectroscopic classification algorithms for lesion discrimination in stereotactic breast biopsies with microcalcifications. J Biophotonics 6:371-81
Cooper, Kimberly L; Oh, Seungeun; Sung, Yongjin et al. (2013) Multiple phases of chondrocyte enlargement underlie differences in skeletal proportions. Nature 495:375-8
Sung, Yongjin; Tzur, Amit; Oh, Seungeun et al. (2013) Size homeostasis in adherent cells studied by synthetic phase microscopy. Proc Natl Acad Sci U S A 110:16687-92
Lau, Condon; Mirkovic, Jelena; Yu, Chung-Chieh et al. (2013) Early detection of high-grade squamous intraepithelial lesions in the cervix with quantitative spectroscopic imaging. J Biomed Opt 18:76013
Soares, Jaqueline S; Barman, Ishan; Dingari, Narahara Chari et al. (2013) Diagnostic power of diffuse reflectance spectroscopy for targeted detection of breast lesions with microcalcifications. Proc Natl Acad Sci U S A 110:471-6
Byun, HeeSu; Hillman, Timothy R; Higgins, John M et al. (2012) Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient. Acta Biomater 8:4130-8
Dingari, Narahara Chari; Barman, Ishan; Myakalwar, Ashwin Kumar et al. (2012) Incorporation of support vector machines in the LIBS toolbox for sensitive and robust classification amidst unexpected sample and system variability. Anal Chem 84:2686-94

Showing the most recent 10 out of 178 publications