Abstract

Existence of left handed DNA (Z-DNA) was discovered and extensively characterized in 1979. Since then there has been considerable research in establishing biological roles for Z-DNA. Recently Rich's group has established that a human protein, ds RNA deminase (dsRAD) has specificity for binding Z-DNA. The goal of this project is to provide direct evidence for the existence of left handed conformation for DNA when it binds to protein ds RAD. Raman spectra of poly(GC) in low salt and high salt solution, control protein, protein/poly (GC) complex have been obtained using 407 nm laser excitation. Raman spectral features of left and right handed DNA conformers have been characterized. By comparing these with the spectra of the protein/ poly (GC) complex, we concluded that DNA exists in left handed conformer when it binds to the ds RAD protein. Additional evidence have been provided by fluorescence and CD spectroscopy.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR002594-15
Application #
6314168
Study Section
Project Start
2000-06-01
Project End
2001-05-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
15
Fiscal Year
2000
Total Cost
$13,842
Indirect Cost

  Institution

Name
Massachusetts Institute of Technology
Department
Type
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02139

  Publications

Shih, Wei-Chuan; Bechtel, Kate L; Rebec, Mihailo V (2015) Noninvasive glucose sensing by transcutaneous Raman spectroscopy. J Biomed Opt 20:051036
Dudzik, Jonathan; Chang, Wen-Chi; Kannan, A M et al. (2013) Cross-linked glucose oxidase clusters for biofuel cell anode catalysts. Biofabrication 5:035009
Sathyavathi, R; Dingari, Narahara Chari; Barman, Ishan et al. (2013) Raman spectroscopy provides a powerful, rapid diagnostic tool for the detection of tuberculous meningitis in ex vivo cerebrospinal fluid samples. J Biophotonics 6:567-72
Dingari, Narahara Chari; Barman, Ishan; Saha, Anushree et al. (2013) Development and comparative assessment of Raman spectroscopic classification algorithms for lesion discrimination in stereotactic breast biopsies with microcalcifications. J Biophotonics 6:371-81
Cooper, Kimberly L; Oh, Seungeun; Sung, Yongjin et al. (2013) Multiple phases of chondrocyte enlargement underlie differences in skeletal proportions. Nature 495:375-8
Sung, Yongjin; Tzur, Amit; Oh, Seungeun et al. (2013) Size homeostasis in adherent cells studied by synthetic phase microscopy. Proc Natl Acad Sci U S A 110:16687-92
Lau, Condon; Mirkovic, Jelena; Yu, Chung-Chieh et al. (2013) Early detection of high-grade squamous intraepithelial lesions in the cervix with quantitative spectroscopic imaging. J Biomed Opt 18:76013
Soares, Jaqueline S; Barman, Ishan; Dingari, Narahara Chari et al. (2013) Diagnostic power of diffuse reflectance spectroscopy for targeted detection of breast lesions with microcalcifications. Proc Natl Acad Sci U S A 110:471-6
Kim, Youngchan; Higgins, John M; Dasari, Ramachandra R et al. (2012) Anisotropic light scattering of individual sickle red blood cells. J Biomed Opt 17:040501
Saha, Anushree; Barman, Ishan; Dingari, Narahara Chari et al. (2012) Precision of Raman spectroscopy measurements in detection of microcalcifications in breast needle biopsies. Anal Chem 84:6715-22

Showing the most recent 10 out of 178 publications