Abstract

This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.We study the biophysics of cell electromotility, the mechanical changes in cell shape driven by the changes in cell membrane potential. Instead of using conventional invasive and point-measurement atomic force microscopy (AFM) technique, we use diffraction phase microscopy (DPM) - a technique developed at G. R. Harrison spectroscopy laboratory, MIT, for imaging sub-nanometer scale motions. With the combination of single electrical frequency excitation, we can non-invasively acquire full-field images of sub-nanometer scale motion of cell electromotility. By studying the electrical frequency response of the cell electromotility, we expect to understand the coupling phenomena between cell's mechanical and electrical circuits.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR002594-23
Application #
7722807
Study Section
Special Emphasis Panel (ZRG1-SBIB-L (40))
Project Start
2008-06-01
Project End
2009-05-31
Budget Start
2008-06-01
Budget End
2009-05-31
Support Year
23
Fiscal Year
2008
Total Cost
$38,106
Indirect Cost

  Institution

Name
Massachusetts Institute of Technology
Department
Internal Medicine/Medicine
Type
Schools of Arts and Sciences
DUNS #
001425594
City
Cambridge
State
MA
Country
United States
Zip Code
02139

  Publications

Shih, Wei-Chuan; Bechtel, Kate L; Rebec, Mihailo V (2015) Noninvasive glucose sensing by transcutaneous Raman spectroscopy. J Biomed Opt 20:051036
Dudzik, Jonathan; Chang, Wen-Chi; Kannan, A M et al. (2013) Cross-linked glucose oxidase clusters for biofuel cell anode catalysts. Biofabrication 5:035009
Sathyavathi, R; Dingari, Narahara Chari; Barman, Ishan et al. (2013) Raman spectroscopy provides a powerful, rapid diagnostic tool for the detection of tuberculous meningitis in ex vivo cerebrospinal fluid samples. J Biophotonics 6:567-72
Dingari, Narahara Chari; Barman, Ishan; Saha, Anushree et al. (2013) Development and comparative assessment of Raman spectroscopic classification algorithms for lesion discrimination in stereotactic breast biopsies with microcalcifications. J Biophotonics 6:371-81
Cooper, Kimberly L; Oh, Seungeun; Sung, Yongjin et al. (2013) Multiple phases of chondrocyte enlargement underlie differences in skeletal proportions. Nature 495:375-8
Sung, Yongjin; Tzur, Amit; Oh, Seungeun et al. (2013) Size homeostasis in adherent cells studied by synthetic phase microscopy. Proc Natl Acad Sci U S A 110:16687-92
Lau, Condon; Mirkovic, Jelena; Yu, Chung-Chieh et al. (2013) Early detection of high-grade squamous intraepithelial lesions in the cervix with quantitative spectroscopic imaging. J Biomed Opt 18:76013
Soares, Jaqueline S; Barman, Ishan; Dingari, Narahara Chari et al. (2013) Diagnostic power of diffuse reflectance spectroscopy for targeted detection of breast lesions with microcalcifications. Proc Natl Acad Sci U S A 110:471-6
Barman, Ishan; Dingari, Narahara Chari; Kang, Jeon Woong et al. (2012) Raman spectroscopy-based sensitive and specific detection of glycated hemoglobin. Anal Chem 84:2474-82
Kalashnikov, Maxim; Choi, Wonshik; Hunter, Martin et al. (2012) Assessing the contribution of cell body and intracellular organelles to the backward light scattering. Opt Express 20:816-26

Showing the most recent 10 out of 178 publications