We are studying the feasibility of using modulated light sources to detect small absorbing objects embedded in a highly scattering medium.JAbsorbing spheres and circular disks of varying size will be submerged in a scattering media (skim milk) and studied in a transillumination geometry. Single linear raster scans in the plane of the sphere were taken and the data, DC intensity, phase, and modulation, were collected. The diffraction pattern of the spheres was appreciably different for the disks demonstrating the volume parameter associated with photon migration. With a 7.2 cm source to detector separation a fairly well localized photon bundle is present. The good localization and deep penetration are characteristics which form the basis for a 3-dimensional image reconstruction method. Since the photon density depends on the local absorption and scattering coefficients a reconstruction of the spectroscopic maps in three dimensions should be possible.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
2P41RR003155-11
Application #
5224550
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
11
Fiscal Year
1996
Total Cost
Indirect Cost
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Liang, Elena I; Mah, Emma J; Yee, Albert F et al. (2017) Correlation of focal adhesion assembly and disassembly with cell migration on nanotopography. Integr Biol (Camb) 9:145-155
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Willenberg, Rafer; Steward, Oswald (2015) Nonspecific labeling limits the utility of Cre-Lox bred CST-YFP mice for studies of corticospinal tract regeneration. J Comp Neurol 523:2665-82
Scarlata, Suzanne; Golebiewska, Urszula (2014) Linking alpha-synuclein properties with oxidation: a hypothesis on a mechanism underling cellular aggregation. J Bioenerg Biomembr 46:93-8
Sharma, Himanshu; Digman, Michelle A; Felsinger, Natasha et al. (2014) Enhanced emission of fluorophores on shrink-induced wrinkled composite structures. Opt Mater Express 4:753-763

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