Abstract

Antifluorescein antibodies and partial protein constructs are being examined in order to isolate the primary conformational states and estimate the magnitude of the conformational substates involved in antibody-hapten interactions. A variety of monoclonal anti-heme antibodies will be examined spectroscopically to characterize the affinity to different porphyrin and heme groups. These measurements will include fluorescence polarization, steady-state fluorescence and CD-measurements. Using different porphyrins as haptens will allow us to determine the antigenic group for each Mab.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR003155-14
Application #
6206050
Study Section
Project Start
1999-08-01
Project End
2000-07-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
14
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of Illinois Urbana-Champaign
Department
Type
DUNS #
041544081
City
Champaign
State
IL
Country
United States
Zip Code
61820

  Publications

Kim, Seong M; Nguyen, Tricia T; Ravi, Archna et al. (2018) PTEN Deficiency and AMPK Activation Promote Nutrient Scavenging and Anabolism in Prostate Cancer Cells. Cancer Discov 8:866-883
Liang, Elena I; Mah, Emma J; Yee, Albert F et al. (2017) Correlation of focal adhesion assembly and disassembly with cell migration on nanotopography. Integr Biol (Camb) 9:145-155
Chen, Hongtao; Gratton, Enrico; Digman, Michelle A (2016) Self-assisted optothermal trapping of gold nanorods under two-photon excitation. Methods Appl Fluoresc 4:035003
Digiacomo, Luca; Digman, Michelle A; Gratton, Enrico et al. (2016) Development of an image Mean Square Displacement (iMSD)-based method as a novel approach to study the intracellular trafficking of nanoparticles. Acta Biomater 42:189-198
Malacrida, Leonel; Astrada, Soledad; Briva, Arturo et al. (2016) Spectral phasor analysis of LAURDAN fluorescence in live A549 lung cells to study the hydration and time evolution of intracellular lamellar body-like structures. Biochim Biophys Acta 1858:2625-2635
Golfetto, Ottavia; Hinde, Elizabeth; Gratton, Enrico (2015) The Laurdan spectral phasor method to explore membrane micro-heterogeneity and lipid domains in live cells. Methods Mol Biol 1232:273-90
Willenberg, Rafer; Steward, Oswald (2015) Nonspecific labeling limits the utility of Cre-Lox bred CST-YFP mice for studies of corticospinal tract regeneration. J Comp Neurol 523:2665-82
Chen, Hongtao; Gratton, Enrico; Digman, Michelle A (2015) Spectral properties and dynamics of gold nanorods revealed by EMCCD-based spectral phasor method. Microsc Res Tech 78:283-93
Scarlata, Suzanne; Golebiewska, Urszula (2014) Linking alpha-synuclein properties with oxidation: a hypothesis on a mechanism underling cellular aggregation. J Bioenerg Biomembr 46:93-8
Sharma, Himanshu; Digman, Michelle A; Felsinger, Natasha et al. (2014) Enhanced emission of fluorophores on shrink-induced wrinkled composite structures. Opt Mater Express 4:753-763

Showing the most recent 10 out of 200 publications