This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We used reconstituted HDL particles to study the efflux of cholesterol from artificial membranes. In order to visualize the removal of cholesterol from individual Giant Unilamellar Vesicles (GUVs), we use dual-channel two-photon fluorescence microscopy and the membrane fluorescent dye Laurdan, The small rHDL particle of 78 diameter showed a higher capability to remove cholesterol than the 96 from POPC GUVs containing 20 % cholesterol.. In the case of the rafts mixture (POPC/Sphingomyelin/cholesterol), the results suggest that the 78 rHDL remove mainly POPC from the liquid phase.
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