Abstract

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In vivo study of Integrin-Linked Kinase (ILK) proteins interactions in C Elegans using FRET microscopy was performed (Breusegem, et al 2002) and the results support the possibility of using FRET microscopy to study the interactions more in detail. In this work, the in vivo study of ILK interactions in adult C Elegans using FRET microscopy is continued and the interactions of the ILK proteins during the formation of muscle structure in the embryonic state will be examined using FRET microscopy and fluorescence correlation spectroscopy.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
2P41RR003155-21
Application #
7357991
Study Section
Special Emphasis Panel (ZRG1-BCMB-E (41))
Project Start
2006-08-29
Project End
2007-07-31
Budget Start
2006-08-29
Budget End
2007-07-31
Support Year
21
Fiscal Year
2006
Total Cost
$16,363
Indirect Cost

  Institution

Name
University of California Irvine
Department
Biomedical Engineering
Type
Schools of Engineering
DUNS #
046705849
City
Irvine
State
CA
Country
United States
Zip Code
92697

  Publications

Kim, Seong M; Nguyen, Tricia T; Ravi, Archna et al. (2018) PTEN Deficiency and AMPK Activation Promote Nutrient Scavenging and Anabolism in Prostate Cancer Cells. Cancer Discov 8:866-883
Liang, Elena I; Mah, Emma J; Yee, Albert F et al. (2017) Correlation of focal adhesion assembly and disassembly with cell migration on nanotopography. Integr Biol (Camb) 9:145-155
Chen, Hongtao; Gratton, Enrico; Digman, Michelle A (2016) Self-assisted optothermal trapping of gold nanorods under two-photon excitation. Methods Appl Fluoresc 4:035003
Digiacomo, Luca; Digman, Michelle A; Gratton, Enrico et al. (2016) Development of an image Mean Square Displacement (iMSD)-based method as a novel approach to study the intracellular trafficking of nanoparticles. Acta Biomater 42:189-198
Malacrida, Leonel; Astrada, Soledad; Briva, Arturo et al. (2016) Spectral phasor analysis of LAURDAN fluorescence in live A549 lung cells to study the hydration and time evolution of intracellular lamellar body-like structures. Biochim Biophys Acta 1858:2625-2635
Chen, Hongtao; Gratton, Enrico; Digman, Michelle A (2015) Spectral properties and dynamics of gold nanorods revealed by EMCCD-based spectral phasor method. Microsc Res Tech 78:283-93
Golfetto, Ottavia; Hinde, Elizabeth; Gratton, Enrico (2015) The Laurdan spectral phasor method to explore membrane micro-heterogeneity and lipid domains in live cells. Methods Mol Biol 1232:273-90
Willenberg, Rafer; Steward, Oswald (2015) Nonspecific labeling limits the utility of Cre-Lox bred CST-YFP mice for studies of corticospinal tract regeneration. J Comp Neurol 523:2665-82
Bonaventura, Gabriele; Barcellona, Maria Luisa; Golfetto, Ottavia et al. (2014) Laurdan monitors different lipids content in eukaryotic membrane during embryonic neural development. Cell Biochem Biophys 70:785-94
Paladino, Simona; Lebreton, Stéphanie; Tivodar, Simona et al. (2014) Golgi sorting regulates organization and activity of GPI proteins at apical membranes. Nat Chem Biol 10:350-357

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