Abstract

This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.This project works with glucose binding proteins conjugated with a fluorophore. The amount of glucose in solution effects the fluorescence quenching. We will be doing scans of the protein in solution using a fluorometer to get an idea of the fluorescence response to different glucose concentrations.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR003155-22
Application #
7600941
Study Section
Special Emphasis Panel (ZRG1-BCMB-E (41))
Project Start
2007-08-01
Project End
2008-07-31
Budget Start
2007-08-01
Budget End
2008-07-31
Support Year
22
Fiscal Year
2007
Total Cost
$2,733
Indirect Cost

  Institution

Name
University of California Irvine
Department
Biomedical Engineering
Type
Schools of Engineering
DUNS #
046705849
City
Irvine
State
CA
Country
United States
Zip Code
92697

  Publications

Kim, Seong M; Nguyen, Tricia T; Ravi, Archna et al. (2018) PTEN Deficiency and AMPK Activation Promote Nutrient Scavenging and Anabolism in Prostate Cancer Cells. Cancer Discov 8:866-883
Liang, Elena I; Mah, Emma J; Yee, Albert F et al. (2017) Correlation of focal adhesion assembly and disassembly with cell migration on nanotopography. Integr Biol (Camb) 9:145-155
Chen, Hongtao; Gratton, Enrico; Digman, Michelle A (2016) Self-assisted optothermal trapping of gold nanorods under two-photon excitation. Methods Appl Fluoresc 4:035003
Digiacomo, Luca; Digman, Michelle A; Gratton, Enrico et al. (2016) Development of an image Mean Square Displacement (iMSD)-based method as a novel approach to study the intracellular trafficking of nanoparticles. Acta Biomater 42:189-198
Malacrida, Leonel; Astrada, Soledad; Briva, Arturo et al. (2016) Spectral phasor analysis of LAURDAN fluorescence in live A549 lung cells to study the hydration and time evolution of intracellular lamellar body-like structures. Biochim Biophys Acta 1858:2625-2635
Chen, Hongtao; Gratton, Enrico; Digman, Michelle A (2015) Spectral properties and dynamics of gold nanorods revealed by EMCCD-based spectral phasor method. Microsc Res Tech 78:283-93
Golfetto, Ottavia; Hinde, Elizabeth; Gratton, Enrico (2015) The Laurdan spectral phasor method to explore membrane micro-heterogeneity and lipid domains in live cells. Methods Mol Biol 1232:273-90
Willenberg, Rafer; Steward, Oswald (2015) Nonspecific labeling limits the utility of Cre-Lox bred CST-YFP mice for studies of corticospinal tract regeneration. J Comp Neurol 523:2665-82
Scarlata, Suzanne; Golebiewska, Urszula (2014) Linking alpha-synuclein properties with oxidation: a hypothesis on a mechanism underling cellular aggregation. J Bioenerg Biomembr 46:93-8
Sharma, Himanshu; Digman, Michelle A; Felsinger, Natasha et al. (2014) Enhanced emission of fluorophores on shrink-induced wrinkled composite structures. Opt Mater Express 4:753-763

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