This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In the fly wing imaginal disc, a Dpp gradient forms from a localized source, where Dpp is secreted from a stripe of anterior cells that is adjacent to the anterior (A)-posterior (P) compartment boundary. The wing disc consists of a lumen and two layers of cells, a peripodial epithelium and a columnar cell layer. Free diffusion in the lumen can not produce the gradient because the secretory transport domains of Dpp have been fused to GFP, and that fusion GFP fails to form a gradient (Entchev et al., 2000). Several transport mechanisms are proposed involving restricted diffusion, planar transcytosis, cytonemes and argosomes. We are going to address this question by tagging Dpp with PhotoActivetable-GFP, and record the movement of a pulse of Dpp-PAGFP with techniques like FCS or single-particle tracking.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR003155-25
Application #
8170980
Study Section
Special Emphasis Panel (ZRG1-BCMB-E (41))
Project Start
2010-08-01
Project End
2011-07-31
Budget Start
2010-08-01
Budget End
2011-07-31
Support Year
25
Fiscal Year
2010
Total Cost
$16,559
Indirect Cost
Name
University of California Irvine
Department
Biomedical Engineering
Type
Schools of Engineering
DUNS #
046705849
City
Irvine
State
CA
Country
United States
Zip Code
92697
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