Abstract

The literature relating to neural tissue preservation, especially the immature spinal cord is very sparse. The ability to pool significant amounts of tissue will depend on the ability to store the samples for specific amounts of time. The purpose of this project is to evaluate hypothermic storage of immature spinal cords tissue in Hypothermosol (HTS). MRI is being evaluated as a possible tool for the assessment of graft survival in vivo. This project is still in its very early stages. Immature spinal cords are dissected out from embryos, and is either used fresh or used at 1, 3 or 7 days after preservation at 4~C in HTS. The specimen is implanted into a 2-mm cavity created in the spinal cords of adult rats, and fixed in place with a CO2 laser. Upon recovery, neurological exams are conducted to determine hind-limb condition. MRI is carried out at 8 weeks, and the animals sacrificed for histological examinations. This study is continuing with examinations on 16 rats being completed thus far. The initial results seem to indicate survival of the graft after 1, 3, and 7 days of hypothermic preservation in HTS. Histological studies have not been completed yet.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR003631-09
Application #
5224634
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
9
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Ramachandran, Suchitra; Meyer, Travis; Olson, Carl R (2016) Prediction suppression in monkey inferotemporal cortex depends on the conditional probability between images. J Neurophysiol 115:355-62
Meyer, Travis; Walker, Christopher; Cho, Raymond Y et al. (2014) Image familiarization sharpens response dynamics of neurons in inferotemporal cortex. Nat Neurosci 17:1388-94
Hall, Nathan; Colby, Carol (2014) S-cone visual stimuli activate superior colliculus neurons in old world monkeys: implications for understanding blindsight. J Cogn Neurosci 26:1234-56
Subramanian, Janani; Colby, Carol L (2014) Shape selectivity and remapping in dorsal stream visual area LIP. J Neurophysiol 111:613-27
Berdyyeva, Tamara K; Olson, Carl R (2014) Intracortical microstimulation of supplementary eye field impairs ability of monkeys to make serially ordered saccades. J Neurophysiol 111:1529-40
Meyer, Travis; Ramachandran, Suchitra; Olson, Carl R (2014) Statistical learning of serial visual transitions by neurons in monkey inferotemporal cortex. J Neurosci 34:9332-7
Hall, Nathan; Colby, Carol (2013) Psychophysical definition of S-cone stimuli in the macaque. J Vis 13:
Leathers, Marvin L; Olson, Carl R (2012) In monkeys making value-based decisions, LIP neurons encode cue salience and not action value. Science 338:132-5
Meyer, Travis; Olson, Carl R (2011) Statistical learning of visual transitions in monkey inferotemporal cortex. Proc Natl Acad Sci U S A 108:19401-6
Berdyyeva, Tamara K; Olson, Carl R (2011) Relation of ordinal position signals to the expectation of reward and passage of time in four areas of the macaque frontal cortex. J Neurophysiol 105:2547-59

Showing the most recent 10 out of 148 publications