This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Dr. Jermin is interested in the trafficking of Kv4.2 in dendrites and spines of CA1 pyramidal neurons in slice cultures, using a combination of two-photon microscopy and various expression techniques. Although organotypic slice cultures have become a widespread culture to investigate the composition of the PSD, very little is know about its ultrastructural organozation. He is interested to fill this gap using electron tomography and begin some trial experiments to see if quantum dots can be used to label endogenous and expressed Kv4.2 (as EGFP fusion) in these slice cultures, as this would potentially allow a correlation of imaging of Kv4.2 channels in living cells and subsequent localization at the ultrastructural level. Finally, he would also like to study synapse formation in these slice cultures combining transgenic technology and expression.
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