A method for measurement of the absolute two-photon absorption cross-section, (, of a molecule in solution has been developed. The ratio of fluorescence induced by pulsed infrared excitation to fluorescence induced by that same excitation doubled in frequency by a non-linear crystal is used to obtain an absolute value for (, eliminating the need for knowledge of the quantum yield, collection efficiency, or concentration. We have used this method in conjunction with our measurements by relative methods of molecules designed by the Marder and Perry groups at Caltech (see Measurement of Molecules with Large Two-Photon Cross-Sections) to have large ( values (5000 GM). Our measurements of Rhodamine B in MeOH agree well with Xu and Webb (1996). Large Two-Photon Cross-Sections) to have large ( values (5000 GM). Our measurements of Rhodamine B in MeOH agree well with Xu and Webb (1996). Chris Xu and Watt W. Webb, """"""""Measurement of two-photon exitation cross-sections of molecular fluorophores with data from 690 to 1050 nm"""""""" in J. Opt. Soc. Am. B 13 (3), 481-491 (1996).

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR004224-13
Application #
6349402
Study Section
Project Start
2000-09-01
Project End
2001-08-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
13
Fiscal Year
2000
Total Cost
$7,966
Indirect Cost
Name
Cornell University
Department
Type
DUNS #
City
Ithaca
State
NY
Country
United States
Zip Code
14850
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