Paraneurons (parafollicular cells), found in the thyroid gland, are endocrine cells, but developmentally related to neurons and moreover can be readily isolated in a pure form for experimental analysis. Serotoninergic neurons cannot similarly be isolated. Work on this cell preparation has revealed that the secretion competent granule interiors experience transient pH drops after cell stimulation. Chloride channels that are regulated by protein kinases C and G are implicated. We are simultaneously imaging 5-HT and a granule pH marker to investigate proposed correlations between 5-HT release and granule pH.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR004224-13
Application #
6349420
Study Section
Project Start
2000-09-01
Project End
2001-08-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
13
Fiscal Year
2000
Total Cost
$36,644
Indirect Cost
Name
Cornell University
Department
Type
DUNS #
City
Ithaca
State
NY
Country
United States
Zip Code
14850
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