Our goals are to acquire simultaneous two-photon images of ALA-induced PpIX and NADH autofluorescence using several well-defined cell lines and normal and tumor tissues of the balb-c mouse. While much is known regarding the heme biosynthesis pathway, recent steady-state fluorescence polarization studies indicate the possibility that the site of PpIX formation may differ in normal and tumor tissues. We propose to address this issue using multiphoton excited NADH autofluorescence and possibly other extrinsic mitochondrial indicators to observe the localization of ALA-induced PpIX in the samples cited above.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR004224-13
Application #
6349440
Study Section
Project Start
2000-09-01
Project End
2001-08-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
13
Fiscal Year
2000
Total Cost
$8,922
Indirect Cost
Name
Cornell University
Department
Type
DUNS #
City
Ithaca
State
NY
Country
United States
Zip Code
14850
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