We have begun prototype development of a new fast and efficient lifetime imaging scheme specifically designed for use with MPM. This novel technique electronically weights the 2PE fluorescent signal by the time of arrival of the fluorescent pulse relative to the excitation pulse by multiplication with a linear ramp. Early arriving photons are weighted proportionally less than late arriving photons to reflect the lifetime of the fluorophore. The ramp acts to provide analog time information to the signal, which is preferable to discrete time binning when low numbers of fluorescent photons are collected. The processing electronics produce an output that will be proportional to the fluorophore lifetime. This technique is similar to that of the fast time gating method, but the sensitivity is potentially much greater for low levels of fluorescence where the statistical fluctuation in the photon arrivals demands narrower binning. Furthermore, integrated electronics to perform the necessary p rocessing are inexpensive and readily available.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR004224-14
Application #
6494073
Study Section
Project Start
2001-09-01
Project End
2002-08-31
Budget Start
Budget End
Support Year
14
Fiscal Year
2001
Total Cost
Indirect Cost
Name
Cornell University
Department
Type
DUNS #
City
Ithaca
State
NY
Country
United States
Zip Code
14850
Migone, Fernando F; Cowan, Robert G; Williams, Rebecca M et al. (2016) In vivo imaging reveals an essential role of vasoconstriction in rupture of the ovarian follicle at ovulation. Proc Natl Acad Sci U S A 113:2294-9
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O'Dell, Ryan S; Ustine, Candida J M; Cameron, David A et al. (2012) Layer 6 cortical neurons require Reelin-Dab1 signaling for cellular orientation, Golgi deployment, and directed neurite growth into the marginal zone. Neural Dev 7:25
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McMullen, Jesse D; Zipfel, Warren R (2010) A multiphoton objective design with incorporated beam splitter for enhanced fluorescence collection. Opt Express 18:5390-8

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