We are in the process of determining the in vivo post-translational modifications of human lens crystallins that occur with cataractogenesis and normal aging. We have developed an HPLC/MS procedure to screen crystallin fractions for post-translational modifications. After a modified crystallin is identified, this fraction is enzymatically digested and analyzed by HPLC/MS to identify the modified peptides, which are then isolated and analyzed by MS/MS to determine the nature and location of the post-translational modification.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR005351-08
Application #
5225021
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
8
Fiscal Year
1996
Total Cost
Indirect Cost
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