This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. NMR Spectroscopy The sample was deuterium-exchanged by lyophilization from D2O, dissolved in 700 uL D2O (99.996% D) containing a trace of sodium 3-(trimethylsilyl)-1-propanesulfonate (DSS). Gradient heteronuclear single quantum coherence (gHSQC) spectra were acquired on a Varian Inova-500 MHz spectrometer at 313 K (40 C). Proton chemical shifts were measured relative to DSS (delta=0.00 ppm). The spectra were recorded with a spectral width in the proton dimension of 3533 Hz and in the carbon dimension of 20115 Hz. The acquisition time was 0.20 s, the number of points was 1414, and 372 increments were collected with 64 scans each. The one-bond C-H coupling constant was set at 140 Hz.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR005351-17
Application #
7358221
Study Section
Special Emphasis Panel (ZRG1-BNP (40))
Project Start
2006-02-01
Project End
2007-01-31
Budget Start
2006-02-01
Budget End
2007-01-31
Support Year
17
Fiscal Year
2006
Total Cost
$1,430
Indirect Cost
Name
University of Georgia
Department
Type
Organized Research Units
DUNS #
004315578
City
Athens
State
GA
Country
United States
Zip Code
30602
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