This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this project is to use a bio-assay directed approach to focus identification work on the most toxicologically important drinking water disinfection by-products (DBPs). To this end, drinking water is being collected from full-scale treatment plants that use chlorine, ozone, chlorine dioxide, and chloramines as disinfectants, and this drinking water is being fractionated according to polarity (through the use of different polarity XAD resins, preparatory liquid chromatography columns, and solvent gradients) and molecular size (through the use of ultrafiltration and size exclusion chromatography). Molecular weight fractions to be studied include <1000, 1000-3000, 3000-5000, 5000-10,000, and >10,000 Da. The bioassays used include a mammalian cell genotoxicity/cytotoxicity assay and a transgenic medaka fish genotoxicity assay. Gas chromatography with high resolution mass spectrometry is being used to identify the lower molecular weight, non-polar drinking water DBPs, and electrospray ionization (ESI)-MS and ESI-MS/MS is being used to identify high molecular weight and highly polar DBPs.
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