The overall goal of this research is to examine the potential of using hyperpolarized gases (Helium & Xenon) for in vivo imaging of air spaces of the normal and diseased lung. MR microscopy has many advantages. Perhaps the most significant is to non-invasively image the live, small animal at microscopic dimensions. Currently, we are able to routinely image the proton or water containing substances of organs and tissues down to 20-30 microns. However, in the case of the lung, we are able to image it with MR with difficulty and only because it has water containing structures conveniently interspersed within it. With hyperpolarized He or Xe gas introduced into the lung, we will be able to image and measure the airspaces of the lung directly. Such capability has enormous potential in animal research of disease models especially for emphysema and asthma. The ability to follow non-invasively in vivo the progression and regression of disease with MR has revolutionized modern diagnosis and therapy. The Center for In Vivo Microscopy is an NIH-funded National Resource charged with extending the application of MR imaging to the microscopic domain and making the tool available to a wide range of basic scientists. The work proposed here will apply directly to models of lung disease. The lung represents a particularly challenging organ system for MR microscopy due to the very limited signal in the lung because of its low density and the constant motion from breathing and cardiac pulsation. Recent work at the Center have demonstrated solutions to both these problems yielding the highest resolution images yet obtained in vivo of the lung. Recent advances in He and Xe gas imaging provide new and exciting possibilities for lung imaging. The techniques propose here will further extend the resolution as well as yield three-dimensional morphometry.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
3P41RR005959-09S1
Application #
6122268
Study Section
Project Start
1999-02-01
Project End
1999-08-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
9
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Duke University
Department
Type
DUNS #
071723621
City
Durham
State
NC
Country
United States
Zip Code
27705
Tang, Xinyan; Jing, Liufang; Richardson, William J et al. (2016) Identifying molecular phenotype of nucleus pulposus cells in human intervertebral disc with aging and degeneration. J Orthop Res 34:1316-26
Hodgkinson, Conrad P; Bareja, Akshay; Gomez, José A et al. (2016) Emerging Concepts in Paracrine Mechanisms in Regenerative Cardiovascular Medicine and Biology. Circ Res 118:95-107
Schmeckpeper, Jeffrey; Verma, Amanda; Yin, Lucy et al. (2015) Inhibition of Wnt6 by Sfrp2 regulates adult cardiac progenitor cell differentiation by differential modulation of Wnt pathways. J Mol Cell Cardiol 85:215-25
Roos, Justus E; McAdams, Holman P; Kaushik, S Sivaram et al. (2015) Hyperpolarized Gas MR Imaging: Technique and Applications. Magn Reson Imaging Clin N Am 23:217-29
He, Mu; Robertson, Scott H; Kaushik, S Sivaram et al. (2015) Dose and pulse sequence considerations for hyperpolarized (129)Xe ventilation MRI. Magn Reson Imaging 33:877-85
Huang, Lingling; Walter, Vonn; Hayes, D Neil et al. (2014) Hedgehog-GLI signaling inhibition suppresses tumor growth in squamous lung cancer. Clin Cancer Res 20:1566-75
Huang, Jing; Guo, Jian; Beigi, Farideh et al. (2014) HASF is a stem cell paracrine factor that activates PKC epsilon mediated cytoprotection. J Mol Cell Cardiol 66:157-64
Yuan, Ying; Gilmore, John H; Geng, Xiujuan et al. (2014) FMEM: functional mixed effects modeling for the analysis of longitudinal white matter Tract data. Neuroimage 84:753-64
He, Mu; Kaushik, S Sivaram; Robertson, Scott H et al. (2014) Extending semiautomatic ventilation defect analysis for hyperpolarized (129)Xe ventilation MRI. Acad Radiol 21:1530-41
van Rhoon, Gerard C; Samaras, Theodoros; Yarmolenko, Pavel S et al. (2013) CEM43°C thermal dose thresholds: a potential guide for magnetic resonance radiofrequency exposure levels? Eur Radiol 23:2215-27

Showing the most recent 10 out of 239 publications