Ankyrin-B is a candidate protein to spatially organize IP3 and ryanodine receptors in striated muscles. We have knocked out the gene encoding ankyrin-B in the mouse by homologous recombination, and are now evaluating its consequences for cardiac development. We have found in isolated cardiomyocytes that the IP3 receptor is mis-sorted, and that patterns of calcium release are grossly abnormal. Ankyrin-B depleted hearts of exhibit reduction in ventricular mass, although their ultra structure is essentially normal. The goals of the proposal are to quantitatively assess the effects of ankyrin-B knock out on the volumetric and microstructural composition of the neonate mouse heart by using both conventional 3D and diffusion tensor MR microscopy.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR005959-10
Application #
6220758
Study Section
Project Start
1999-09-15
Project End
2000-08-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
10
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Duke University
Department
Type
DUNS #
071723621
City
Durham
State
NC
Country
United States
Zip Code
27705
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