This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Demonstration of MR histology in neurologic dvelopment has spurred interest from neuroscientists, developmental biologists, and toxicologists. Numerous studies in these fields will benefit from ability to study mouse brain in 3 dimensions through critical stages of development. But studies will require normative comparison data. This project will acquire a 4-dimensional image array of developing mouse brain. More specifically, we will image the developing brain of FVB mouse at 2-day intervals ranging from 2 days through at least 24 days. - Refine methods for active staining and 3D scanning in the mouse brain - Acquire 4-dimensional data set (3 dimensions in space; 1 in time) covering critical stages of development in the FVB mouse brain (post natal day 2--24). This image set will provide critical data to our interest in developing tools (Core D) for visualizing time critical phenomena in 3 spatial dimensions - Develop an atlas of normative data to provide landmarks for following neurologic development with quantitative morphometry - Provide insight into the mechanisms determining contrast in actively stained brains as myellination takes place.
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