This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We propose to study two infant specimens: one skull containing suture and one cervical spine level. Both specimens would undergo the highest resolution MDCT, micro CT, and histologic preparation. The specimens will be sectioned and stained for a histological study to determine precisely where the transition from bone to suture occurs. Both sets of CT data will be reconstructed using the AMIRA software in collaboration with the Duke Visualization Technology Group (for examples of theses reconstructions, please see http://hyoid.lsrc.duke.edu/~rwn/res/pedimesh/index.htm). A study group consisting of biomedical engineering, radiology, and pathology personnel would review the histology data to determine the correlates with both MDCT and micro CT. Histologic preparation and MDCT techniques could be made available upon request. For micro CT, the examinations would be performed under the guidance of the designee(s) of Al Johnson, Ph.D, Director, Center for In-vivo microscopy. It would be of great value to match the standard MDCT, micro-CT, and the histological slides with micro-CT. This would allow us to compare thresholding techniques for both imaging modalities with the gold standard of histology. If the micro CT can better differentiate these tissues, then we will propose to use it to scan a selection of pediatric heads and necks for use in developing next generation of models for injury research. It is expected that grant funding will be secured for this investigation.
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