This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The use of imaging modalities for prostate cancer diagnosis and treatment monitoring remains limited regarding its real value. The current data suggested that an introduction of MRI with endorectal coil, being more advanced techinque, also have significant limitation for accurate visualization of primary or recurrent prostate cancer. We are going to do a compehensive investigation of primary prostate cancer in murine prostate cancer model and human prostatectomized specimens after radical prostatectomy. Mouse prostate carcinoma model Growth of the mouse prostate cancer cell line RM-9 has been previous described and will be provided by Dr. T.C. Thompson from the Baylor College of medicine (1). Attached RM-9 cells will be trypsinized after growthing to approximately 90% confluency and washed once with phosphate-buffered saline and then resuspended in EMEM medium (Biofluid). The cell member will be adjust into 106 cells/ml and mixed with equal volume of Matrigel (Becton-Dickinson, Franklin Lakes, NJ). An inoculum of 105 in 0.2 ml mix will be injected subcutaneously (s.c.) into flank of each mouse. The two-dimensional tumor base area will be measured with calipers every other day, starting on day 7 up to 28 days. In average it takes 5-7 days after grafting in order to develop advanced prostate cancer. Four to six week-old male C57BL/J6 mice will be used. All mice were maintained in facilities approved by the American Association for Accreditation of Laboratory Animal Care, and all animal studies were conducted in accordance with the principles and procedures outlined in the NIH s Guide for the Care and Use of Laboratory Animals. Two arms of mice will be used (14 mice in each group, total-28 mice) - tested and control (just with s.c. injection of saline into the same site- flank of each mouse) groups. MRI microscopy will be performed on 7th day after inoculation. Then all mice will be harvested with removal of prostate for molecular biology and histology evaluation in order to validate imaging data.
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