Abstract

Ion fluxes and ATPase activity are studied in intact cells,resealed ghosts and cell-free membranes, and 31P NMR spectroscopyis used to follow energetics in whole cells. Effects oftemperature on these measurements are studied in red cells of coldsensitive (human) and insensitive (ground squirrel) species. Wehave demonstrated that species differences in the effects oftemperature on free intracellular magnesium can account for muchif not all of the difference in temperature sensitivity of theNa/K pump. Studies using 19F NMR and a fluorinated calciumindicator have now begun in order to measure changes inintracellular free calcium as a function of

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
2P41RR005964-06A1
Application #
5225181
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Li, Li; Palencia, Andrés; Lukk, Tiit et al. (2013) Leucyl-tRNA synthetase editing domain functions as a molecular rheostat to control codon ambiguity in Mycoplasma pathogens. Proc Natl Acad Sci U S A 110:3817-22
Li, Li; Boniecki, Michal T; Jaffe, Jacob D et al. (2011) Naturally occurring aminoacyl-tRNA synthetases editing-domain mutations that cause mistranslation in Mycoplasma parasites. Proc Natl Acad Sci U S A 108:9378-83
Sallam, Khalid Ibrahim (2007) Antimicrobial and antioxidant effects of sodium acetate, sodium lactate, and sodium citrate in refrigerated sliced salmon. Food Control 18:566-575
Sallam, Khalid Ibrahim (2007) Chemical, sensory and shelf life evaluation of sliced salmon treated with salts of organic acids. Food Chem 101:592-600
Sallam, Kh I; Samejima, K (2004) Microbiological and chemical quality of ground beef treated with sodium lactate and sodium chloride during refrigerated storage. Lebenson Wiss Technol 37:865-871
Moser, Kevin W; Georgiadis, John G (2004) Extraction and validation of correlation lengths from interstitial velocity fields using diffusion-weighted MRI. Magn Reson Imaging 22:257-68
Sallam, Kh I; Ishioroshi, M; Samejima, K (2004) Antioxidant and antimicrobial effects of garlic in chicken sausage. Lebenson Wiss Technol 37:849-855
Sallam, Khalid Ibrahim; Samejima, Kunihiko (2004) Effects of Trisodium Phosphate and Sodium Chloride Dipping on the Microbial Quality and Shelf Life of Refrigerated Tray-packaged Chicken Breasts. Food Sci Biotechnol 13:425-429
Wang, Steven J; Brechbiel, Martin; Wiener, Erik C (2003) Characteristics of a new MRI contrast agent prepared from polypropyleneimine dendrimers, generation 2. Invest Radiol 38:662-8
Swain, R A; Harris, A B; Wiener, E C et al. (2003) Prolonged exercise induces angiogenesis and increases cerebral blood volume in primary motor cortex of the rat. Neuroscience 117:1037-46

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