This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.A nano-meter size pore, a so-called nanopore, can be manufactured in thin inorganic membranes. The most important application for nanopores is DNA sequencing: under the influence of an electric field, DNA translocates through the nanopore, producing electrical signals characteristic of the sequence and length of the DNA strand. Current synthetic nanopores can not reach single-base resolution yet; however, they are among the most promising technologies for cheap DNA sequencing. The Resource has been working in close collaboration with electrical engineers (Gregory Timp and Jean-Pierre Leburton) to understand the physics of synthetic nanopores and improve their resolution. Atomic-scale modeling was carried out in three directions: (i) genotyping with synthetic nanopore; (ii) stretching/ unzipping DNA hairpins with a synthetic nanopore; (iii) sensing DNA sequence with a nanopore capacitor; (iv) modeling of ionic current through silica nanopores (www.ks.uiuc.edu/Research/nanopore/).

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR005969-19
Application #
7723596
Study Section
Special Emphasis Panel (ZRG1-BCMB-E (40))
Project Start
2008-08-01
Project End
2009-07-31
Budget Start
2008-08-01
Budget End
2009-07-31
Support Year
19
Fiscal Year
2008
Total Cost
$47,367
Indirect Cost
Name
University of Illinois Urbana-Champaign
Department
Type
Organized Research Units
DUNS #
041544081
City
Champaign
State
IL
Country
United States
Zip Code
61820
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Decker, Karl; Page, Martin; Aksimentiev, Aleksei (2017) Nanoscale Ion Pump Derived from a Biological Water Channel. J Phys Chem B 121:7899-7906
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Vermaas, Josh V; Taguchi, Alexander T; Dikanov, Sergei A et al. (2015) Redox potential tuning through differential quinone binding in the photosynthetic reaction center of Rhodobacter sphaeroides. Biochemistry 54:2104-16
Belkin, Maxim; Chao, Shu-Han; Jonsson, Magnus P et al. (2015) Plasmonic Nanopores for Trapping, Controlling Displacement, and Sequencing of DNA. ACS Nano 9:10598-611
Shen, Rong; Han, Wei; Fiorin, Giacomo et al. (2015) Structural Refinement of Proteins by Restrained Molecular Dynamics Simulations with Non-interacting Molecular Fragments. PLoS Comput Biol 11:e1004368

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