Abstract

Fluorescence emission anisotropies, r, and mean lifetimes of 4-dimethylamino-omega-diphenylphosphinyl-trans-styrene (DDPS) were measured in n-butanol at different temperatures from 20 to 80xC for one- and two-photon excitation. When increasing the temperature, the lifetime decreased from 32 to 12 ps, the r values, however, remained constant (0.315 and 0.445 for one- and two-photon excitation, respectively) and were found to be temperature independent. For DDPS in glycerol at -5xC, where rotational diffusion does not occur during the excited state lifetime, the limiting emission anisotropies were r_0 = 0.382 and r_0 - 0.545 for one- and two-photon excitation, respectively, very little differing from the fundamental emission anisotropies r_f - 2/5 and r_f = 4/7. The small differences r_f - r_0 can be due to vibrations performed after excitation by the DDPS molecule as a result of the initial shock.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR008119-04
Application #
5225697
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Piao, Wenji; Shirey, Kari Ann; Ru, Lisa W et al. (2015) A Decoy Peptide that Disrupts TIRAP Recruitment to TLRs Is Protective in a Murine Model of Influenza. Cell Rep 11:1941-52
Hartman, Kira G; McKnight, Laura E; Liriano, Melissa A et al. (2013) The evolution of S100B inhibitors for the treatment of malignant melanoma. Future Med Chem 5:97-109
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Zhang, Jian; Badugu, Ramachandram; Lakowicz, Joseph R (2008) Fluorescence Quenching of CdTe Nanocrystals by Bound Gold Nanoparticles in Aqueous Solution. Plasmonics 3:3-11
Zhang, Jian; Fu, Yi; Chowdhury, Mustafa H et al. (2007) Metal-enhanced single-molecule fluorescence on silver particle monomer and dimer: coupling effect between metal particles. Nano Lett 7:2101-7
Zhang, Jian; Fu, Yi; Chowdhury, Mustafa H et al. (2007) Single-Molecule Studies on Fluorescently Labeled Silver Particles: Effects of Particle Size. J Phys Chem C Nanomater Interfaces 112:18
Zhang, Jian; Fu, Yi; Lakowicz, Joseph R (2007) Single cell fluorescence imaging using metal plasmon-coupled probe. Bioconjug Chem 18:800-5
Zhang, Jian; Fu, Yi; Lakowicz, Joseph R (2007) Emission Behavior of Fluorescently Labeled Silver Nanoshell: Enhanced Self-Quenching by Metal Nanostructure. J Phys Chem C Nanomater Interfaces 111:1955-1961
Zhang, Jian; Lakowicz, Joseph R (2007) Metal-enhanced fluorescence of an organic fluorophore using gold particles. Opt Express 15:2598-606
Fu, Yi; Lakowicz, Joseph R (2007) Single-Molecule Studies of Enhanced Fluorescence on Silver Island Films. Plasmonics 2:1-4

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