The Center for Fluorescence Spectroscopy (CFS) is a multi-user facility which is focused on the advanced biomedical applications of fluorescence. The CFS was first supported by the National Center for Research Resources in April of 1993 when it received four years of funding. In this competitive renewal request we propose development of the following core technologies: 1. Development of long-lifetime (100 ns to 5 mus) and love-wavelength probes for use in fluorescence spectroscopy, microscopy and sensing. We will emphasize development of metal-ligand complexes which are conjugatable to macromolecules, or which are sensitive to Ca2+, Mg2+, K+, Na+ and pH, and which display high quantum yields, analyte-sensitive lifetimes, and/or high anisotropy. 2. Two- and three-photon excitation of biochemical fluorophores, characterization of decay times, photostability, excitation and anisotropy spectra, and studies of alkane fluorescence with two-photon excitation. 3.Development of the experimental and theoretical aspects of light quenching, which allows control of the excited state population by long wavelength light pulses. 4. Development of the methodology of fluorescence lifetime imaging microscopy, including software and instrumentation, using xenopus embryos, cos, PC12, 293 and neonatal heart cells as model systems. 5. Development of cell-by-cell lifetime measurements of intracellular ions in flow cytometry. 6. Continued development of software for time domain (TD), frequency- domain (FD) and FLIM. The CFS will continue to work with collaborators while developing the core technologies, and to provide access to TD and TD measurement for many internal and external collaborators and users. Dissemination of information is accomplished by the presence of our CFS booth at national meetings, organization of bi-annual national meetings, the Journal of Fluorescence and the newly founded Journal of Biomedical Optics. Additionally, each year the CFS teaches an intensive week-long course on the principles of time resolved fluorescence.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR008119-07
Application #
2901462
Study Section
Special Emphasis Panel (ZRG3-BPC (04))
Program Officer
Farber, Gregory K
Project Start
1993-04-01
Project End
2002-03-31
Budget Start
1999-04-01
Budget End
2000-03-31
Support Year
7
Fiscal Year
1999
Total Cost
Indirect Cost
Name
University of Maryland Baltimore
Department
Biochemistry
Type
Schools of Medicine
DUNS #
003255213
City
Baltimore
State
MD
Country
United States
Zip Code
21201
Piao, Wenji; Shirey, Kari Ann; Ru, Lisa W et al. (2015) A Decoy Peptide that Disrupts TIRAP Recruitment to TLRs Is Protective in a Murine Model of Influenza. Cell Rep 11:1941-52
Hartman, Kira G; McKnight, Laura E; Liriano, Melissa A et al. (2013) The evolution of S100B inhibitors for the treatment of malignant melanoma. Future Med Chem 5:97-109
Fu, Yi; Zhang, Jian; Lakowicz, Joseph R (2009) Highly efficient detection of single fluorophores in blood serum samples with high autofluorescence. Photochem Photobiol 85:646-51
Zhang, Jian; Badugu, Ramachandram; Lakowicz, Joseph R (2008) Fluorescence Quenching of CdTe Nanocrystals by Bound Gold Nanoparticles in Aqueous Solution. Plasmonics 3:3-11
Zhang, Jian; Fu, Yi; Chowdhury, Mustafa H et al. (2007) Metal-enhanced single-molecule fluorescence on silver particle monomer and dimer: coupling effect between metal particles. Nano Lett 7:2101-7
Zhang, Jian; Fu, Yi; Chowdhury, Mustafa H et al. (2007) Single-Molecule Studies on Fluorescently Labeled Silver Particles: Effects of Particle Size. J Phys Chem C Nanomater Interfaces 112:18
Zhang, Jian; Fu, Yi; Lakowicz, Joseph R (2007) Single cell fluorescence imaging using metal plasmon-coupled probe. Bioconjug Chem 18:800-5
Zhang, Jian; Fu, Yi; Lakowicz, Joseph R (2007) Emission Behavior of Fluorescently Labeled Silver Nanoshell: Enhanced Self-Quenching by Metal Nanostructure. J Phys Chem C Nanomater Interfaces 111:1955-1961
Zhang, Jian; Lakowicz, Joseph R (2007) Metal-enhanced fluorescence of an organic fluorophore using gold particles. Opt Express 15:2598-606
Fu, Yi; Lakowicz, Joseph R (2007) Single-Molecule Studies of Enhanced Fluorescence on Silver Island Films. Plasmonics 2:1-4

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