Abstract

Molecular dynamic simulations reveal an exposed heme edge that has not been observed in any crystal or NMR structure of cytochrome b5. This cleft is likely to be of functional importance for recognition of a redox partner for mediating direct electron transfer for some complexes. In order to study dynamics of the cleft breathing we initiated a steady-state fluorescence study of single Trp-22. Acrylamide quenching of the single tryptophan rat cytochrome b5 and its various disufide mutants was examined by ultrafast time-resolved spectroscopy. In order to probe dynamics of the putative cleft, an additional tryptophan has been placed on the surface (S18W). Comparison of lifetime distribution and anisotropy decays of wild-type and mutant with Trp-18 may provide new information about mobility of the protein surface in the cleft region of the protein.?

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR008119-07
Application #
6122880
Study Section
Project Start
1999-04-01
Project End
2000-03-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
7
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of Maryland Baltimore
Department
Type
DUNS #
003255213
City
Baltimore
State
MD
Country
United States
Zip Code
21201

  Publications

Piao, Wenji; Shirey, Kari Ann; Ru, Lisa W et al. (2015) A Decoy Peptide that Disrupts TIRAP Recruitment to TLRs Is Protective in a Murine Model of Influenza. Cell Rep 11:1941-52
Hartman, Kira G; McKnight, Laura E; Liriano, Melissa A et al. (2013) The evolution of S100B inhibitors for the treatment of malignant melanoma. Future Med Chem 5:97-109
Fu, Yi; Zhang, Jian; Lakowicz, Joseph R (2009) Highly efficient detection of single fluorophores in blood serum samples with high autofluorescence. Photochem Photobiol 85:646-51
Zhang, Jian; Badugu, Ramachandram; Lakowicz, Joseph R (2008) Fluorescence Quenching of CdTe Nanocrystals by Bound Gold Nanoparticles in Aqueous Solution. Plasmonics 3:3-11
Zhang, Jian; Lakowicz, Joseph R (2007) Metal-enhanced fluorescence of an organic fluorophore using gold particles. Opt Express 15:2598-606
Fu, Yi; Lakowicz, Joseph R (2007) Single-Molecule Studies of Enhanced Fluorescence on Silver Island Films. Plasmonics 2:1-4
Ray, Krishanu; Szmacinski, Henryk; Enderlein, Joerg et al. (2007) Distance dependence of surface plasmon-coupled emission observed using Langmuir-Blodgett films. Appl Phys Lett 90:251116
Chowdhury, Mustafa H; Malyn, Stuart N; Aslan, Kadir et al. (2007) First Observation of Surface Plasmon-Coupled Chemiluminescence (SPCC). Chem Phys Lett 435:114-118
Zhang, Jian; Fu, Yi; Chowdhury, Mustafa H et al. (2007) Enhanced Forster Resonance Energy Transfer on Single Metal Particle. 2. Dependence on Donor-Acceptor Separation Distance, Particle Size, and Distance from Metal Surface. J Phys Chem C Nanomater Interfaces 111:11784-11792
Zhang, Jian; Fu, Yi; Lakowicz, Joseph R (2007) Luminescent images of single gold nanoparticles and their labeling on silica beads. Opt Express 15:13415-20

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