We examined the time-resolved and steady-state fluorescence quenching of N-acetyl-L-tryptophanamide. (NATA) by acrylamide and iodide, over a range of viscosities in propylene glycol. The quenching of NATA by acrylamide and iodide results in heterogeneity of the intensity decay which increases with the quencher concentration. We attribute the complex decay of NATA to transient effects in diffusion and the nature of the fluorophore-quencher interaction. Consideration of both the frequency-domain and steady-state data demonstrate that the quenching rate depends exponentially on the fluorophore-quencher distance, indicating the validity of a model which include a quenching constant which depend exponentially on distance. Such data on the distance dependence of quenching is important for understanding quenching of native proteins.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR008119-09
Application #
6444705
Study Section
Project Start
2001-04-01
Project End
2002-03-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
9
Fiscal Year
2001
Total Cost
$293,069
Indirect Cost
Name
University of Maryland Baltimore
Department
Type
DUNS #
003255213
City
Baltimore
State
MD
Country
United States
Zip Code
21201
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